Promega Corporation

BCIP/NBT Color Development Substrate (5-bromo-4-chloro-3-indolyl-phosphate/nitro blue...

BCIP/NBT Color Development Substrate (5-bromo-4-chloro-3-indolyl-phosphate/nitro blue tetrazolium)

BCIP (5-bromo-4-chloro-3-indolyl-phosphate) is used in conjunction with NBT (nitro blue tetrazolium) for the colorimetric detection of alkaline phosphatase activity. Each vial of BCIP is supplied with a vial of NBT.

Preparation of Substrates to Detect Alkaline Phosphatase: For every 5ml of alkaline phosphatase buffer (100mM Tris-HCl [pH 9.0], 150mM NaCl, 1mM MgCl2), add 33μl NBT and 16.5μl BCIP. Add the NBT first, mix, add the BCIP, and mix again. Use within 1 hour, and discard any unused solution.

Concentration: BCIP (50mg/ml) in 100% dimethylformamide; NBT (50mg/ml) in 70% dimethylformamide....

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BCIP/NBT Color Development Substrate

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BCIP/NBT Color Development Substrate

 Components

  • NBT

    S380C1 x 2.5ml
  • BCIP

    S381C1 x 1.25ml
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This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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1.25/2.5ml
- S3771 € 95,00 Add to cart


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Storage Conditions

Store at either 4°C or –20°C.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

S3771 For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

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