Promega Corporation

UGT Activity Assays

The UGT-Glo™ Assay provides a luminescent method for measuring UDP glucuronosyltransferase (UGT) activity. The UGT-Glo™ Assay is designed to measure UGT activity from a variety of sources, such as microsomes containing recombinantly expressed enzymes or microsomal preparations derived from mammalian tissues, and to test the effects of various chemicals on UGT activity.

The assay involves incubating UGT with a proluciferin substrate; a portion of the substrate gets conjugated with UDP, while the remainder is unmodified. Upon the addition of d-Cysteine, the unconjugated proluciferin is converted into luciferin an...

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UGT-Glo™ Assay

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UGT-Glo™ Assay

 Components

  • UDPGA, 50mM

    V209A1 x 640μl
  • UGT Buffer, 5X

    V210A1 x 1.6ml
  • UGT Multienzyme Substrate

    V213A1 x 10μl
  • d-Cysteine, 100X

    V251A1 x 100μl
  • UGT1A4 Substrate

    V260A1 x 10μl
  • Luciferin Detection Reagent

    V859A1 x 1 each
  • Reconstitution Buffer

    V865A1 x 10ml
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


200 assays
- V2081 € 300,00 Add to cart

UGT-Glo™ Assay

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UGT-Glo™ Assay

 Components

  • UDPGA, 50mM

    V209B1 x 3.2ml
  • UGT Buffer, 5X

    V210B1 x 8ml
  • UGT Multienzyme Substrate

    V213B1 x 50μl
  • d-Cysteine, 100X

    V251B1 x 500μl
  • UGT1A4 Substrate

    V260B1 x 50μl
  • Luciferin Detection Reagent

    V859B1 x 1 each
  • Reconstitution Buffer

    V865B1 x 50ml
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


1,000 assays
- V2082 € 1.090,00 Add to cart

UGT-Glo™ UGT1A1 Screening System

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UGT-Glo™ UGT1A1 Screening System

 Components

  • UDPGA, 50mM

    V209A1 x 640μl
  • UGT Buffer, 5X

    V210A1 x 1.6ml
  • UGT Multienzyme Substrate

    V213A1 x 10μl
  • UGT1A1 Microsomes

    V241A1 x 160μl
  • d-Cysteine, 100X

    V251A1 x 100μl
  • UGT1A4 Substrate

    V260A1 x 10μl
  • Control Membranes

    V476A1 x 100μl
  • Luciferin Detection Reagent

    V859A1 x 1 each
  • Reconstitution Buffer

    V865A1 x 10ml
200 assays
- V2120 € 545,00 Add to cart

UGT-Glo™ UGT1A1 Screening System

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UGT-Glo™ UGT1A1 Screening System

 Components

  • UDPGA, 50mM

    V209B1 x 3.2ml
  • UGT Buffer, 5X

    V210B1 x 8ml
  • UGT Multienzyme Substrate

    V213B1 x 50μl
  • UGT1A1 Microsomes

    V241B1 x 800μl
  • d-Cysteine, 100X

    V251B1 x 500μl
  • UGT1A4 Substrate

    V260B1 x 50μl
  • Control Membranes

    V476A1 x 100μl
  • Luciferin Detection Reagent

    V859B1 x 1 each
  • Reconstitution Buffer

    V865B1 x 50ml
1,000 assays
- V2121 € 2.180,00 Add to cart

UGT-Glo™ UGT2B7 Screening System

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UGT-Glo™ UGT2B7 Screening System

 Components

  • UDPGA, 50mM

    V209A1 x 640μl
  • UGT Buffer, 5X

    V210A1 x 1.6ml
  • UGT Multienzyme Substrate

    V213A1 x 10μl
  • UGT2B7 Microsomes

    V245A1 x 160μl
  • d-Cysteine, 100X

    V251A1 x 100μl
  • UGT1A4 Substrate

    V260A1 x 10μl
  • Control Membranes

    V476A1 x 100μl
  • Luciferin Detection Reagent

    V859A1 x 1 each
  • Reconstitution Buffer

    V865A1 x 10ml
200 assays
- V2130 € 545,00 Add to cart

UGT-Glo™ UGT2B7 Screening System

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UGT-Glo™ UGT2B7 Screening System

 Components

  • UDPGA, 50mM

    V209B1 x 3.2ml
  • UGT Buffer, 5X

    V210B1 x 8ml
  • UGT Multienzyme Substrate

    V213B1 x 50μl
  • UGT2B7 Microsomes

    V245B1 x 800μl
  • d-Cysteine, 100X

    V251B1 x 500μl
  • UGT1A4 Substrate

    V260B1 x 50μl
  • Control Membranes

    V476A1 x 100μl
  • Luciferin Detection Reagent

    V859B1 x 1 each
  • Reconstitution Buffer

    V865B1 x 50ml
1,000 assays
- V2131 € 2.180,00 Add to cart


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Storage Conditions

Store UGT enzymes and Control Membranes at –70°C. Store remaining components at –20°C.

For product intended use please see Patents & Disclaimers tab.

Conversion of UGT Multienzyme Substrate by UGT enzymes.Figure 1. Conversion of UGT Multienzyme Substrate by UGT enzymes.

UGT enzymes attach a glucuronic acid moiety to the proluciferin substrate. During the detection step, the unconjugated proluciferin is converted to luciferin by cyclization with D-cysteine and then converted into light by luciferase. Glucuronidated proluciferin does not get converted into luciferin, and thus does not produce light. Light output is inversely proportional to UGT enzymatic activity.

UGT enzymes attach a glucuronic acid moiety to the proluciferin substrate. During the detection step, the unconjugated proluciferin is converted to luciferin by cyclization with D-cysteine and then converted into light by luciferase. Glucuronidated proluciferin does not get converted into luciferin, and thus does not produce light. Light output is inversely proportional to UGT enzymatic activity.

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Use Restrictions

V2081, V2082, V2120, V2121, V2130, V2131 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

V2081, V2082, V2120, V2121, V2130, V2131 U.S. Pat. Nos. 6,602,677, 7,241,584 and 8,030,017 and other patents and patents pending.

V2081, V2082, V2120, V2121, V2130, V2131 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.

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