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The CellTiter-Blue® Cell Viability Assay provides a homogeneous, fluorescent method for monitoring cell viability. The assay is based on the ability of living cells to convert a redox dye (resazurin) into a fluorescent end product (resorufin). Nonviable cells rapidly lose metabolic capacity and thus do not generate a fluorescent signal. The homogeneous assay procedure involves adding the single reagent directly to cells cultured in serum-supplemented medium. After an incubation step, data are recorded using either a plate-reading fluorometer (preferred) or spectrophotometer.
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For more information, see the Protocols & Applications Guide.
Each G8080 system provides sufficient reagents to perform 1,000 assays in a 96-well format or 4,000 assays in a 384-well format when the recommended volumes are used. Likewise, each G8081 system provides sufficient reagents to perform 5,000 and 20,000 assays; each G8082 system provides sufficient reagents to perform 50,000 and 200,000 assays.
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CellTiter-Blue® Cell Viability Assay
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This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.
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Store frozen at –20°C protected from light.
For product intended use please see Patents & Disclaimers tab.
G8080, G8081, G8082 For Research Use Only. Not for Use in Diagnostic Procedures.
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viernes, 26 de julio de 2013 by javier mendez (hospital josep trueta girona)
a la hora de hacer cultivos es muy importante valorar la viabilidad, y el cell blue realmente me ha servido mucho a la hora de hacer pruebas de IC50 de farmacos,
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