Promega Corporation

CellTiter-Glo® One Solution Assay

The CellTiter-Glo® One Solution Assay is a homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, which indicates the presence of metabolically active cells. This frozen, ready-to-use format is based on the original CellTiter-Glo® Luminescence Cell Viability Assay chemistry and eliminates the need to combine buffer with lyophilized substrate when preparing reagent. The CellTiter-Glo® Assay is designed for use with multiwell-plate formats, making it ideal for automated high-throughput screening (HTS) in 96- to 1536-well format, and cell prolife...

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CellTiter-Glo® One Solution Assay
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CellTiter-Glo® One Solution Assay  Components

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  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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100ml
- G8461 Please Enquire
CellTiter-Glo® One Solution Assay
Close Window

CellTiter-Glo® One Solution Assay  Components

Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


500ml
- G8462 Please Enquire


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Storage Conditions

Store the CellTiter-Glo® One Solution Assay below –10°C. CellTiter-Glo® One Solution Assay can be stored at 4°C for 48 hours or at 22°C for 10 hours with ~10–12% loss of activity. CellTiter-Glo® One Solution Assay can withstand two additional freeze-thaw cycles after the first thaw, with approximately 10% loss of activity with each additional freeze-thaw cycle.

For product intended use please see Patents & Disclaimers tab.

Flow diagram showing preparation and use of the CellTiter-Glo® One Solution.Figure 1. Flow diagram showing preparation and use of the CellTiter-Glo® One Solution.
Cell number correlates with luminescent output.Figure 2. Cell number correlates with luminescent output.

A direct relationship exists between luminescence measured with the CellTiter-Glo® One Solution Assay and the number of cells in culture over three orders of magnitude. Serial twofold dilutions of Jurkat cells were made in RPMI 1640 with 10% FBS and plated in three formats using automated dispensing equipment. Volumes plated were 100µl of cells per well in a 96-well plate (Panel A), 10µl of cells per well in low-volume 384-well plate (Panel B) and 4µl of cells per well in a 1536-well plate (Panel C). An equivalent volume of CellTiter-Glo® One Solution was dispensed into each well. Luminescence was recorded 10 minutes after addition of CellTiter-Glo® One Solution. There is a linear relationship (r2 > 0.99) between luminescent signal and cell number in each plate format.

Use Restrictions

G8461, G8462 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

G8461, G8462 U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.

G8461, G8462 U.S. Pat. No. 7,741,067 and other patents and patents pending.

G8461, G8462 U.S. Pat. Nos. 6,602,677, 7,241,584 and 8,030,017 and other patents and patents pending.

G8461, G8462 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.

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