Promega Corporation

PPase-2A

Protein Phosphatase-2A (PPase-2A) is a serine/threonine phosphatase isolated from human red blood cells. It is isolated as the heterodimer of 60kDa (A) and 36kDa (C) subunits. It has the ability to dephosphorylate the α-subunit of phosphorylase kinase. With its 36–38kDa catalytic subunit, PPase-2A has broad substrate specificity and may play a regulatory role in DNA replication, transcription, protein synthesis, mitosis and glycogen metabolism. PPase-2A is stimulated in vitro by basic proteins such as protamine, histones and polylysine. The enzyme is inhibited by several environmental toxins and tumor promoters su...

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PPase-2A

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PPase-2A

 Components

  • PPase-2A

    V631A1 x 25u
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  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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25u
- V6311 € 640,00 Add to cart


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Storage Buffer

20mM MOPS (pH 7.5), 150mM NaCl, 1mM MgCl2, 1mM EGTA, 0.1mM MnCl2, 1mM DTT, 0.1mg/ml serum albumin, 60mM 2-mercaptoethanol, 50% glycerol.

Storage Conditions

Store at –20°C.

Unit Definition

One unit is defined as the amount of PPase-2A required to release 1nmol of phosphate per minute at 30°C using p-nitrophenyl phosphate (PNPP) as the substrate.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

V6311 For Research Use Only. Not for Use in Diagnostic Procedures.

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