Promega Corporation

ProFluor® Ser/Thr PPase Assay

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The ProFluor® Ser/Thr PPase Assay measures purified serine/threonine protein phosphatase activity in a multiwell plate format and involves "add-mix-read" steps only—ideal for high-throughput applications. The assay works with protein phosphatase 1 (PP1), PP2A, PP2B and PP2C. The assay begins with a standard phosphatase reaction performed with a provided phosphorylated bisamide rhodamine 110 peptide substrate (S/T PPase R110 Substrate) and Control AMC Substrate that serves as a control for compounds that may inhibit the protease reaction. Following the phosphatase reaction, a termination buffer containing a proteas...

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ProFluor® Ser/Thr PPase Assay

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ProFluor® Ser/Thr PPase Assay

 Components

  • Stabilizer Reagent

    V306A1 x 12μl
  • Protease Reagent

    V309A1 x 240μl
  • S/T PPase R110 Substrate

    V324A1 x 12μl
  • Control AMC Substrate

    V325A1 x 12μl
  • MgCl2, 1M

    V330A1 x 1ml
  • MnCl2, 20mM

    V331A1 x 1ml
  • CaCl2, 100mM

    V332A1 x 1ml
  • NiCl2, 100mM

    V333A1 x 1ml
  • 5X Reaction Buffer B

    V334A1 x 6ml
  • 5X Termination Buffer B

    V335A1 x 5ml
4 plate
- V1260 € 1.425,00 Add to cart

ProFluor® Ser/Thr PPase Assay

Close Window

ProFluor® Ser/Thr PPase Assay

 Components

  • Stabilizer Reagent

    V306B1 x 24μl
  • Protease Reagent

    V309B1 x 480μl
  • S/T PPase R110 Substrate

    V324B1 x 24μl
  • Control AMC Substrate

    V325B1 x 24μl
  • MgCl2, 1M

    V330A1 x 1ml
  • MnCl2, 20mM

    V331A1 x 1ml
  • CaCl2, 100mM

    V332A1 x 1ml
  • NiCl2, 100mM

    V333A1 x 1ml
  • 5X Reaction Buffer B

    V334B1 x 12ml
  • 5X Termination Buffer B

    V335B1 x 10ml
8 plate
- V1261 € 2.135,00 Add to cart


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Storage Conditions

Store the entire system at –20°C. Protect the S/T PPase R110 Substrate and Control AMC Substrate from light.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

V1260, V1261 For Research Use Only. Not for Use in Diagnostic Procedures.

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