Promega Corporation

Wizard® Plus SV Minipreps DNA Purification Systems

Special Offer: Switch to the Vacuum Format and get a Free Welch® Vacuum Pump! For a limited time Wizard® Plus SV Minipreps Start-Up Kits are available. Purchase a Wizard® Plus SV Minipreps DNA Purification System and a Vac-Man® Vacuum Manifold, and get a free Welch® Vacuum Pump! Each vacuum pump bundle is available in 3 formats. If you are not sure which format is right for your geographic area, please contact your Promega representative or an authorized Promega distributor.

The Wizard® Plus SV Minipreps DNA Purification System, a silica membrane-based system, provides a simple and reliable method for r...

Expand to Read More »

  • Share
  • Print
  • Email
  • Prices valid for customers of Promega Biotech Iberica only
Product Size Conc. Catalog # *List Price Order QTY Add to Cart

Wizard® Plus SV Minipreps DNA Purification System

Close Window

Wizard® Plus SV Minipreps DNA Purification System

 Components

  • Wizard® SV Minicolumns

    A129A1 x 50/pk
  • Collection Tubes

    A130A1 x 50/pk
  • Column Wash Solution (CWA)

    A131B1 x 20ml
  • Alkaline Protease Solution

    A144A1 x 550μl
  • Neutralization Solution (NSB)

    A148B1 x 30ml
  • Cell Resuspension Solution (CRA)

    A711P1 x 20ml
  • Cell Lysis Solution (CLA)

    A712P1 x 20ml
  • Nuclease-Free Water

    P119E1 x 13ml
Close Window
  • This product can be added to a

  • Helix Cabinet

This product is available through the Promega Helix onsite stocking program in a room temperature cabinet. The program offers numerous convenient solutions to meet your lab's needs. The Helix Cabinet is available in two sizes: 7.9 cubic ft. or 25 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


50 preps
- A1330 € 95,00 Add to cart

Wizard® Plus SV Minipreps DNA Purification System

4.00
Close Window

Wizard® Plus SV Minipreps DNA Purification System

 Components

  • Wizard® SV Minicolumns

    A129B1 x 250/pk
  • Collection Tubes

    A130B1 x 250/pk
  • Column Wash Solution (CWA)

    A131C1 x 100ml
  • Alkaline Protease Solution

    A144B1 x 3ml
  • Neutralization Solution (NSB)

    A148C1 x 100ml
  • Cell Resuspension Solution (CRA)

    A711N1 x 75ml
  • Cell Lysis Solution (CLA)

    A712N1 x 75ml
  • Nuclease-Free Water

    P119C1 x 25ml
Close Window
  • This product can be added to a

  • Helix Cabinet

This product is available through the Promega Helix onsite stocking program in a room temperature cabinet. The program offers numerous convenient solutions to meet your lab's needs. The Helix Cabinet is available in two sizes: 7.9 cubic ft. or 25 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


250 preps
- A1460 € 390,00 Add to cart

Wizard® Plus SV Minipreps DNA Purification System

5.00
Close Window

Wizard® Plus SV Minipreps DNA Purification System

 Components

  • Wizard® SV Minicolumns

    A129B4 x 250/pk
  • Collection Tubes

    A130B4 x 250/pk
  • Column Wash Solution (CWA)

    A131C4 x 100ml
  • Alkaline Protease Solution

    A144B4 x 3ml
  • Neutralization Solution (NSB)

    A148C4 x 100ml
  • Cell Resuspension Solution (CRA)

    A711N4 x 75ml
  • Cell Lysis Solution (CLA)

    A712N4 x 75ml
  • Nuclease-Free Water

    P119C2 x 25ml
1,000 preps
- A1465 € 1.255,00 Add to cart

Wizard® Plus SV Minipreps DNA Purification System + Vacuum Adapters

3.00
Close Window

Wizard® Plus SV Minipreps DNA Purification System + Vacuum Adapters

 Components

  • Wizard® SV Minicolumns

    A129A1 x 50/pk
  • Collection Tubes

    A130A1 x 50/pk
  • Column Wash Solution (CWA)

    A131B1 x 20ml
  • Vacuum Adapters

    A13311 x 20 each
  • Alkaline Protease Solution

    A144A1 x 550μl
  • Neutralization Solution (NSB)

    A148B1 x 30ml
  • Cell Resuspension Solution (CRA)

    A711P1 x 20ml
  • Cell Lysis Solution (CLA)

    A712P1 x 20ml
  • Nuclease-Free Water

    P119E1 x 13ml
Close Window
  • This product can be added to a

  • Helix Cabinet

This product is available through the Promega Helix onsite stocking program in a room temperature cabinet. The program offers numerous convenient solutions to meet your lab's needs. The Helix Cabinet is available in two sizes: 7.9 cubic ft. or 25 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


50 preps
- A1340 € 118,00 Add to cart

Wizard® Plus SV Minipreps DNA Purification System + Vacuum Adapters

Close Window

Wizard® Plus SV Minipreps DNA Purification System + Vacuum Adapters

 Components

  • Wizard® SV Minicolumns

    A129B1 x 250/pk
  • Collection Tubes

    A130B1 x 250/pk
  • Column Wash Solution (CWA)

    A131C1 x 100ml
  • Vacuum Adapters

    A13311 x 20 each
  • Alkaline Protease Solution

    A144B1 x 3ml
  • Neutralization Solution (NSB)

    A148C1 x 100ml
  • Cell Resuspension Solution (CRA)

    A711N1 x 75ml
  • Cell Lysis Solution (CLA)

    A712N1 x 75ml
  • Nuclease-Free Water

    P119C1 x 25ml
Close Window
  • This product can be added to a

  • Helix Cabinet

This product is available through the Promega Helix onsite stocking program in a room temperature cabinet. The program offers numerous convenient solutions to meet your lab's needs. The Helix Cabinet is available in two sizes: 7.9 cubic ft. or 25 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


250 preps
- A1470 € 410,00 Add to cart

Wizard® Plus SV Minipreps Start-Up Kit, 110V Electrical (750 preps, manifold and free vacuum pump)

Close Window

Wizard® Plus SV Minipreps Start-Up Kit, 110V Electrical (750 preps, manifold and free vacuum pump)

 Components

  • Vacuum Adapters

    A13311 x 20 each
  • Wizard® Plus SV Minipreps DNA Purification System

    A14603 x 250 preps
  • Welch Vacuum Pump for North America Electrical

    A67201 x 1 each
  • Vac-Man® Laboratory Vacuum Manifold, 20-sample capacity

    A72311 x 1 each
1 each
- A6760 Please Enquire

Wizard® Plus SV Minipreps Start-Up Kit, 220V Electrical (750 preps, manifold and free vacuum pump)

Close Window

Wizard® Plus SV Minipreps Start-Up Kit, 220V Electrical (750 preps, manifold and free vacuum pump)

 Components

  • Vacuum Adapters

    A13311 x 20 each
  • Wizard® Plus SV Minipreps DNA Purification System + Vacuum Adapters

    A14703 x 250 preps
  • Welch Vacuum Pump for Continental Europe Electrical

    A67221 x 1 each
  • Vac-Man® Laboratory Vacuum Manifold, 20-sample capacity

    A72311 x 1 each
1 each
- A6762 € 1.475,00 Add to cart

Wizard® Plus SV Minipreps Start-Up Kit, 230V Electrical (750 preps, manifold and free vacuum pump)

Close Window

Wizard® Plus SV Minipreps Start-Up Kit, 230V Electrical (750 preps, manifold and free vacuum pump)

 Components

  • Vacuum Adapters

    A13311 x 20 each
  • Wizard® Plus SV Minipreps DNA Purification System + Vacuum Adapters

    A14703 x 250 preps
  • Welch Vacuum Pump for Japan Electrical

    A67241 x 1 each
  • Vac-Man® Laboratory Vacuum Manifold, 20-sample capacity

    A72311 x 1 each
1 each
- A6764 Please Enquire


Available Separately

PRODUCT SIZE CONC CATALOG # *List Price ORDER QTY. Add to Cart

Column Wash Solution (CWA)

Close Window

Column Wash Solution (CWA)

 Components

Close Window
  • This product can be added to a

  • Helix Cabinet

This product is available through the Promega Helix onsite stocking program in a room temperature cabinet. The program offers numerous convenient solutions to meet your lab's needs. The Helix Cabinet is available in two sizes: 7.9 cubic ft. or 25 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


185ml
- A1311 € 110,00 Add to cart

Alkaline Protease Solution

Close Window

Alkaline Protease Solution

 Components

  • Alkaline Protease Solution

    A144B1 x 3ml
Close Window
  • This product can be added to a

  • Helix Cabinet

This product is available through the Promega Helix onsite stocking program in a room temperature cabinet. The program offers numerous convenient solutions to meet your lab's needs. The Helix Cabinet is available in two sizes: 7.9 cubic ft. or 25 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


3ml
- A1441 € 51,00 Add to cart

Vacuum Adapters

Close Window

Vacuum Adapters

 Components

20 each
- A1331 € 33,00 Add to cart

You Also May Be Interested In

Storage Conditions

Store at 22–25°C.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

A1330, A1460, A1465, A1340, A1470, A6760, A6762, A6764, A1441, A1331 For Research Use Only. Not for Use in Diagnostic Procedures.
A1311 For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Patents - Disclaimers

A1330, A1460, A1465, A1340, A1470 U.S. Pat. No. 5,981,235 and other patents and patents pending.

Wizard Plus SV Minipreps DNA Purification System

4.00

martes, 18 de septiembre de 2012 by Isabel García, Ciudad Real

Muy buen rendimiento de purificación, en cantidad y calidad. Protocolo rápido y sencillo.

Wizard Plus SV Minipreps DNA Purification System

5.00

martes, 11 de febrero de 2014 by Marga Saiz, CBM Madrid

Sistema de extracción de DNA plasmídico rápido, limpio y muy económico. Buen rendimiento comparable con otros sistemas más sofisticados.

Wizard Plus SV Minipreps DNA Purification System + Vacuum Adapters

0.00

martes, 24 de septiembre de 2013 by María Jurado Pueyo. Servicio Técnico de Promega, España

El rendimiento esperado con el Wizard® Plus SV Miniprep es de 3.5-5µg de DNA plasmídico cuando se utiliza el plásmido pGEM®/células DH5α™/1.5ml de LB, o de 1.5-3µg de DNA plasmídico cuando se utiliza un plásmido de bajo nº de copias como pALTER®-1 (en este caso partiendo de 10ml de cultivo). En nuestra experiencia, es frecuente que con métodos caseros que implican extracciones con fenol se obtenga mayor cantidad de DNA. No obstante, las ventajas del kit Wizard® Plus SV Miniprep son la obtención de un ácido nucléico de mayor calidad (debido entre otras cosas al tratamiento con proteasa alcalina), la rapidez del método, la posibilidad de ser utilizado por centrifugación o por vacío, la no utilización de fenol ni de precipitaciones, etc.Por otro lado, existen varios factores que pueden afectar al rendimiento de la purificación: el nº de copias del plásmido, el tamaño del inserto, el medio de cultivo, la capacidad de unión del sistema de purificación, la cepa bacteriana, etc. Como se describe en el manual técnico del kit, es muy importante utilizar cultivos frescos y añadir antibióticos tanto al medio sólido como al medio líquido, utilizar plásmidos de alto número de copia y cuantificar el DNA plasmídico mediante electroforesis. También es fundamental asegurarse de que el pellet se ha resuspendido completamente antes de la lisis. Por último, cuando se emplean plásmidos de alto nº de copia, se recomienda un máximo de 5ml de cultivo, para no exceder el límite de la columna.

Prefer a different language?

Your country is set to Spain. Your language is set to español. Please select the language that will best suit your needs:

This is correct, continue to site »

I need additional help

It appears that you have Javascript disabled. Our website requires Javascript to function correctly. For the best browsing experience, please enable Javascript.