Special Offer: Switch to the Vacuum Format and get a Free Welch® Vacuum Pump! For a limited time PureYield™ RNA Midiprep Start-Up Kits are available. Purchase a PureYield™ RNA Midiprep System and a Vac-Man® Vacuum Manifold, and get a free Welch® Vacuum Pump! Each vacuum pump bundle is available in 3 formats. If you are not sure which format is right for your geographic area, please contact your Promega representative or an authorized Promega distributor.
The PureYield™ RNA Midiprep System isolates intact, pure total RNA from essentially any sample type for use in a wide range of applications. The use of...
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The PureYield™ RNA Midiprep System isolates intact, pure total RNA from essentially any sample type for use in a wide range of applications. The use of a novel Clearing Agent enables the rapid purification of total RNA with undetectable levels of genomic DNA contamination without using DNase. A novel combination of reagents, membranes and protocol leads to yields of up to 1mg of total RNA without organic solvents, protease digestions or alcohol precipitations. One kit can be used to isolate pure total RNA from a wide variety of sample types, such as tissues, cultured cells, bacteria, yeast, plants and blood. The protocol also can be adapted for other sample types.
Commonly used methods provide total RNA that is contaminated with genomic DNA. This contamination can interfere with sensitive methods, such as real-time RT-PCR and microarray analysis. The PureYield™ RNA Midiprep System avoids this problem by selectively removing the genomic DNA prior to total RNA purification. The eluted total RNA is free of detectable DNA and ready for use in sensitive downstream applications.
The system has been designed for use with centrifugation or vacuum (e.g., the Vac-Man® Laboratory Vacuum Manifold) formats.
The Eluator™ Vacuum Elution Device is used to elute nucleic acids from PureYield™ Midiprep or Maxiprep columns. It consists of two pieces, a blue base and a clear column assembly. The base interfaces with a Vacuum Manifold that contains Luer-Lok® fittings, such as the Vac-Man® Laboratory Vacuum Manifold (Cat.# A7231), and holds a 1.5ml tube to capture the eluted nucleic acids. The column assembly accepts PureYield™ Midiprep or Maxiprep columns.
The Eluator™ Device eliminates the requirement for a centrifuge with a swinging bucket rotor for nucleic acid purification, simplifying and speeding purification protocols.
Sample types processed using the PureYield™ RNA Midiprep System.
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PureYield™ RNA Midiprep System
PureYield™ Binding Columns
PureYield™ Clearing Columns
RNA Lysis Buffer (RLA)
RNA Wash Solution (RWA)
RNA Dilution Buffer (RDB)
Clearing Agent (CAA)
This product can be added to a
This product is available through the Promega Helix onsite stocking program in a room temperature cabinet. The program offers numerous convenient solutions to meet your lab's needs. The Helix Cabinet is available in two sizes: 7.9 cubic ft. or 25 cubic ft.
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PureYield™ RNA Midiprep Start-Up Kit, 110V Electrical (100 preps, manifold, 4 elution devices and free vacuum pump)
Eluator™ Vacuum Elution Device
Welch Vacuum Pump for North America Electrical
Vac-Man® Laboratory Vacuum Manifold, 20-sample capacity
PureYield™ RNA Midiprep Start-Up Kit, 220V Electrical (100 preps, manifold, 4 elution devices and free vacuum pump)
Welch Vacuum Pump for Continental Europe Electrical
PureYield™ RNA Midiprep Start-Up Kit, 230V Electrical (100 preps, manifold, 4 elution devices and free vacuum pump)
Welch Vacuum Pump for Japan Electrical
Red Blood Cell Lysis Solution (CLB)
Store the RNA Lysis Buffer (RLA) with added β-Mercaptoethanol (BME) at 4°C. Store all other components at 22–25°C.
For product intended use please see Patents & Disclaimers tab.
Elution is completed using vacuum pressure with a Vac-Man® Laboratory Manifold in less than one minute directly into 1.5ml centrifuge tubes.
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