Quality DNA purification systems, amplification master mixes, methylation-sensitive enzymes, and reporter assays for epigenetics research.
Bisulfite Conversion
The new MethylEdge™ Bisulfite Conversion System provides an efficient method to rapidly perform bisulfite conversion, giving you more intact, converted DNA for downstream applications.
PCR, qPCR and RT-qPCR
Improve PCR performance in techniques such as bisulfite-specific PCR with the GoTaq® polymerase family of master mixes.
Methylation-Specific Restriction Enzymes
We offer high-quality, dependable enzymes for cloning and DNA analysis. Promega methylation-specific restriction enzymes include: HpaII, MboI, MspI, and Sau3AI.
Luciferase-Based Methylation Detection
We offer FuGENE® HD transfection reagent and optimized luciferase genes and vectors designed for use in reporter assays. Use these tools to measure methylation status and methylation regulators precisely and with high sensitivity.
This chapter of the Protocols and Applications Guide provides protocols and information regarding heritable changes in gene expression that arise from changes in chromosomes without alteration of DNA sequence.
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