Predictive Biology – Simplified
Kinase profiling has become a crucial, but costly, necessity for developing targeted kinase inhibitors. The new Kinase Enzyme Systems provide the enzyme, substrate, buffer, and ADP-Glo™ reagents in an integrated, easy-to-use protocol for quantifying the activity of over 170 kinases, including lipid kinases.
- ADP-Glo™ Kinase Assay
- Kinase Enzyme Systems
- Lipid Kinase Assay Reagents
ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is converted into light by Ultra-Glo™ Luciferase. The luminescent signal positively correlates with kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.
Each Kinase Enzyme System is comprised of a recombinant human kinase, substrate, buffer and supplemental reagents optimized for use with the ADP-Glo Kinase Assay. An Application Note for each KES is available that gives specific information on how to use the reagents with ADP-Glo, which minimizes to amount of optimization required.
Promega lipid kinase enzymes, substrates and detection systems provide a complete set of reagents for performing phosphoinositide lipid kinase (PIK) reactions using a luminescent ADP-detection platform, the ADP-Glo™ Kinase Assay. The reagents include purified human recombinant proteins of Class I PI3Ks, optimized reaction buffer and ready-to-use lipid kinase substrates.