ApoTox-Glo™ Triplex Assay
Predictive Mechanism of Toxicity Determination
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Measure viability, cytotoxicity, and apoptosis in the same sample well: Accurately determine mechanism of cell death in the same sample well, save time and use less cells.
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Easily implement: The assay follows a simple sequential "add-mix-measure" protocol.
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Normalize data with a built-in control: The ratio of the number of live cells/number of dead cells is independent of cell number and normalizes data, making it more comparable well-to-well, plate-to-plate, and day-to-day.
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Flexible and easily automated: Component volumes can be scaled to meet throughput needs, and reagents are robust for automation in 96- to 1536-well plate formats.
Predictive Mechanism of Toxicity Determination
Download our Cell Based Assays video.
Closed Format: MP4
Open Format: OGG
Measure viability, cytotoxicity and apoptosis in the same sample well
Primary Necrosis
- Ionomycin treatment of Jurkat cells for 6 hours.
- Dose-dependent decrease in viability
- No caspase-3/7 activation
Apoptosis
- Staurosporine treatment of Jurkat cells for 6 hrs.
- Dose-dependent decrease in viability
- Increase in caspase-3/7 activation
Easily implement and adapt to multiwell formats
Simple add-mix-measure protocol and scalable assay reagents make the assay easy to implement and adaptable for 96- to 1536-well formats.
Normalize data
The ratio of number of live cells to number of dead cells is independent of cell number and normalizes the data. This normalization makes results more comparable well-to-well, plate-to-plate and day-to-day.