A modular, easy to use and cost effective multimode reader for Luminescence, Fluorescence, and Absorbance
The GloMax®-Multi+ replaces the Turner BioSystems Modulus II Microplate Multimode Reader. Same instrument... new look!
New and Improved Instinct™ Software
- GloMax Detection Instruments provide the highest luminescence sensitivity, broadest dynamic range, and best value for researchers worldwide.
- Integrated with Promega's industry-leading bioluminescence assays for Cell Health and Gene Reporter Assays
Our New and Improved Instinct™ Software is more robust than ever, including:
- Easier data export directly into Microsoft® Excel
- Ability to export graphs into the final reports
- PC and Macintosh computer compatible
- Added pre-programmed protocols for quick assay setup
- Flexible for European regional data settings
Click Here to request a free product demonstration and learn how GloMax can help solve your scientific questions.
AUTHENTIMAX™ SOFTWARE for GLOMAX®-MULTI+ (Optional)
The Code of Federal Regulations, Title 21, Part 11 (21 CFR Part 11) includes the US Federal Drug Administration requirements for storing and protecting electronic records, and applying electronic signatures to electronic files. AuthentiMax™ Software for GloMax®-Multi+ is an optional Microsoft Windows® PC (not Mac) based software that supports user authentication and document integrity, and creates an audit trail while applying electronic signatures to electronic records.
OVERVIEW
The GloMax®-Multi+ Microplate Multimode Reader is designed for today's life science laboratory. In addition to giving performance equivalent to that of single-mode instruments, the GloMax®-Multi+ blends user-friendly operation with easy data handling and flexible purchasing options. The result of this design is an instrument with superior performance that is easy to use, is affordable, and can be customized to your laboratory's needs.
PERFORMANCE
The GloMax®-Multi+ Microplate Multimode Reader combines the superior performance expected from single-mode instruments with the functionality of multiple modes. To achieve industry-leading performance, the GloMax®-Multi+ Microplate is designed with optical channels dedicated to each individual technology. Unlike other multimode systems, readings taken with the GloMax®-Multi+ Microplate are not degraded by indirect fiber-optic transmission or crowded optical channels. Dedicated optical channeling ensures that the GloMax®-Multi+ Microplate provides sensitivity and dynamic range on par with the highest performing single-mode instruments.
EASE OF USE
The GloMax®-Multi+ Microplate Multimode Reader is designed to be put into use straight from the box, without the need for special training. The built-in computer eliminates the connectivity hassles, cost, and space requirements of running an external computer. Assay set-up on the GloMax®-Multi+ is effortless with several flexible set-up options to choose from.
AFFORDABLE MODULARITY
The GloMax®-Multi+ Microplate Multimode Reader is a modular instrument that easily fits into most budgets. Purchase the technology or modes that you need now and add onto the system later as your needs expand. For example, the GloMax®-Multi+ can be purchased as a luminometer, then fluorescence and/or absorbance modules can be purchased and added later.Installations take less than ten minutes and can be done right in your lab using just the Allen wrench provided with the module. In addition, after installing a new module, you will not need to download and install new software. The GloMax®-Multi+ instantly detects the newly installed module(s) and will automatically adjust screens, protocols, and options.
DATA HANDLING
To transfer data to a PC, simply copy the data from the GloMax®-Multi+ then move the USB stick (included) to your computer. Analyze your data when and where you find it most convenient.
TEMPERATURE CONTROL (Optional)
The GloMax®-Multi+ may be purchased with an optional heater, allowing precise temperature control from 2° above ambient temperature to 45° C.
SHAKING
The GloMax®-Multi+ is configured with a factory-installed shaker that allows either linear or orbital shaking.
MICROPLATE FORMAT
Accepts 6, 12, 24, 48, 96, and 384-well plates conforming to the SBS plate standard.
COMPUTER INTERFACE
For those labs that would prefer to operate the GloMax®-Multi+ through an external PC, the PC Software contains all of the same ease-of-use features that are available via the instrument's built-in touch screen.
INJECTORS
Single or dual auto injectors are available for flash-based luminescence applications or dual-reporter assays. Each injector system has a volume range of 25 - 200 µl in 5µl increments for 6- to 96-well plate formats. Injectors are controlled using the touch screen and fluidics wizard. PRIME and FLUSH commands provide easy maintenance and a REVERSE PURGE command saves valuable reagents.
COLOR TOUCH SCREEN
The GloMax®-Multi+ combines a 6.6" color touch screen display with an onboard Windows-based computer.
INTUITIVE USER INTERFACE
Setting up a run and retrieving data are fast and simple while maintaining the flexibility needed for advanced or custom protocols.
PROTOCOL COMPOSER
Complex protocols can easily be developed by combining multiple technologies into one experiment. Save time and reagents by testing for both luminescence and fluorescence, or fluorescence and absorbance, in one run.
INSTINCT SOFTWARE
With Instinct™ software, included with GloMax Multi+ instruments, you can perform data analysis right on the touch screen. With a few simple button selections, label your data, choose an analysis model and see the analyzed results after the data is collected.
TOUCH SCREEN SELECTION
The Windows-based computer built into the GloMax-Multi+ Microplate Multimode Reader offers dynamic user-interface capabilities. Simply choose from three options on the instrument touch screen to start setting up a run.
CREATE A NEW PROTOCOL
Use the NEW PROTOCOL wizard to create a customized protocol. The wizard guides you step-by-step through choosing read parameters, injection methods, and plate wells to read, and in saving your protocol for future use.
CHOOSE A PRELOADED PROTOCOL
Choose SELECT PROTOCOL to access to the most popular assays from common reagent suppliers, or instantly access previously saved user-customized protocols.
TOTAL INSTRUMENT CONTROL
From the INSTRUMENT CONTROL screen, simply touch the parameter to change or customize all settings necessary to obtain the perfect read for your application. Once your parameters are set, you can save your settings for future use.
INSTINCT™ SOFTWARE PLATE MAP
Use the advanced plate map features to select a curve fit model, create sample groups, and label wells.
INSTINCT SOFTWARE RESULTS
Results screens display raw data, calculated concentrations and graphs for instant review of your data.
PROTOCOL COMPOSER
From the INSTRUMENT CONTROL screen, move a parameter into the Protocol Composer list to create multi-parameter protocols. Complex protocols can easily be developed by combining multiple technologies into one experiment. Save time and reagents by testing for both luminescence and fluorescence, or fluorescence and absorbance, in one run.
DETECTION MODES
Luminescence Module
(Factory Installed)
>8 Logs Dynamic Range
Dual-masking System Reduces Crosstalk
3 x 10-21 Moles Luciferase Detection or 1 x 10-18 moles ATP
To achieve the sensitivity of a dedicated luminometer, the luminescence channel is separated from other measurement technologies and positioned directly above the sample well. These conditions maximize light capture for the best possible sensitivity. In addition, a low-noise photomultiplier tube ensures that collected light is not compromised in any way. This design makes the GloMax-Multi+ Microplate Multimode Reader between 10 to 1000 times more sensitive than competing multimode luminometers. In addition to high sensitivity, the GloMax®-Multi+ boasts greater than 8 logs of dynamic range, eliminating the need to dilute samples or manage detector-driven gain changes. The photomultiplier tube automatically adjusts for the optimum reading of bright or dim samples. This means that the GloMax®-Multi+ is capable of achieving a reading range 2 - 3 logs more than that of competing multimode luminometers.
| Luminescence Injector/Application Chart |
| Recommended Injectors |
Applications |
| 2 |
Dual Reporter Assays |
| 0 |
Steady-Glo® Luciferase Assays |
| 1 |
Flash Glow Luciferase Assays |
| 1 |
Cell Viability/ATP Assays |
| 1 |
Kinetics Assays |
Fluorescence Module
(User Installable)
Epifluorescent Detection (top reading)
Easy Optical Kit Switching
Wavelength-matched LEDs Ensure High Sensitivity
To achieve high performance, the Fluorescence Module uses powerful light-emitting diodes (LEDs) as excitation sources. LEDs have very specific light-output profiles that closely match the excitation profiles of commonly used fluorescent molecules. LED usage increases sensitivity by fully exciting the fluorophore and reducing non-specific light leakage, a common problem when using broad-spectrum light sources. Five standard optical kits are available to measure the most popular fluorophores. In addition, custom optical kits can be readily made for non-standard applications. Optical kits can be easily exchanged in seconds and built-in software ensures that the installed optical kit matches the selected protocol. Designed as a user-installable module, you can either buy the Fluorescence Module with your GloMax®-Multi+ instrument or add it to your system later when you are ready to run fluorescence experiments.
To inquire about availability of custom optical kits click here to contact us. Please, note we cannot guarantee assay performance when using custom optical kits.
| Fluorescence Module Optical Kit Application Chart |
| Fluorescence Optical Kit |
Typical Applications |
UV Optical Kit
Ex: 365 nm
Em: 410 - 460 nm |
DNA Quantification (Hoechst dye), Enzyme Activity (4-methyl-umbelliferone) |
Blue Optical Kit
Ex: 490 nm
Em: 510 - 570 nm |
DNA Quantitation, RNA Quantitation, Protein Labeling (Fluorescein), Protein Quantitation, Gene Expression (EGFP, rAcGFP) |
Green Optical Kit
Ex: 525 nm
Em: 580 - 640 nm |
Nucleotide or Protein Labeling (Rhodamine, Cy3), Enzyme Activity (Rhodamine) |
Red Optical Kit
Ex: 625 nm
Em: 660 - 720 nm |
Nucleotide or Protein Labeling (Cy5), RNA Quantitation |
AFC Optical Kit
Ex: 405 nm
Em: 495 - 505 nm |
Cell Viability, Cytotoxicity, and Apoptosis Multiplex Assays |
Absorbance Module
(User Installable)
UV-Visible or Visible Modules
Flexible Filter System
Reads Single or Dual Wavelengths
The GloMax-Multi+ Microplate Multimode Reader provides you with two different options for making photometric measurements. A Visible Absorbance Module (450 - 750 nm) or a UV-Visible Absorbance Module (200 - 1100 nm). Both modules are user installable, so there is no downtime or need for a service call.
Both the Visible and UV-Visible Absorbance Modules come factory-installed with four common filters: 450, 560, 600, and 750 nm wavelengths. The UV-Visible module also comes with a 260 and 280 nm filter. In addition, you can customize either module by inserting a filter of your choice into either of two removable filter paddles.
To inquire about availability of custom optical kits click here to contact us. Please, note we cannot guarantee assay performance when using custom optical kits.
| Absorbance Module Filter Application Chart |
| Wavelength |
Applications |
| 260 |
DNA and RNA Quantification |
| 280 |
Protein Quantitation |
| 450 |
ELISA, QuantiCleave™ Protease Assay |
| 560 |
BCA™ Protein Assays |
| 600 |
Bradford Protein Assays, Coomassie Blue Protein Assays, PeroXOquant™ Quantitative Peroxide Assay |
| 750 |
Lowry Protein Assay |
SPECIFICATIONS
| GLOMAX®-Multi+ |
| Available Detection Modes |
Luminescence, Fluorescence, Visible Absorbance, UV-Visible Absorbance |
| Read Type |
Glow, Flash, Kinetic, Repeat |
| Sample Format* |
6, 12, 24, 48, 96, 384-well plates |
| Shaking** |
Four speeds (150, 300, 500 rpm, Variable) linear and orbital modes |
| Temperature Control** |
2 ºC above ambient temperature to 45 ºC +/- 0.75 ºC. Order Instrument Cat #E9032 |
| User Interface |
Built-in PC, touch screen navigation and operation |
| Data Output*** |
PC compatible ASCII text file format exported to USB flash drive, or connect to PC (not included) through USB or Serial Port |
| External PC Requirements (optional) |
Windows XP, Vista & Windows 7 |
| Power |
100 - 240 VAC, 50 - 60 Hz |
| Auto Shutoff |
Touch screen hibernates after 15 min of inactivity |
| Dimensions |
21" D x 17.3" W x 12.83" H (53 cm D x 44 cm W x 32.6 cm H) |
| Weight |
~35 lbs (~16 kg) |
| Operating Temperature |
60 - 85 ºF (15 - 30 ºC) |
| Warranty |
One year parts and labor |
| Approvals |
CE |
| LUMINESCENCE |
| Detector |
Head-on photomultiplier tube (PMT) for photon counting |
| Spectral Range |
350 - 650 nm |
| Peak Wavelength |
420 nm |
| Detection Limit |
3 x 10-21 moles of luciferase or 1 x 10-18 moles of ATP |
| Linear Dynamic Range |
>8 logs |
| Crosstalk |
5 x 10-5 |
| SINGLE INJECTOR SYSTEM |
| Number of Injectors |
One injector |
| Dispense Volume Range |
Selectable between 25 - 200 μl in 5 μl increments for 6 to 96-well plate formats. |
| Waste Tray Volume |
~50 ml |
| DUAL INJECTOR SYSTEM |
| Number of Injectors |
Two injectors |
| Dispense Volume Range |
Selectable between 25 - 200 μl in 5 μl increments for 6 to 96-well plate formats. |
| Waste Tray Volume |
~50 ml |
| FLUORESCENCE |
| Light Source |
Wavelength-matched LED |
| Detector |
PIN-photodiode |
| Read Position |
Top reading |
| Wavelength Selection |
Snap-in Fluorescence Optical Kits |
| Wavelengths |
UV (Ex 365 nm: Em 410 - 460 nm), Blue (Ex 490 nm: Em 510 - 570 nm), Green (Ex 525 nm: Em 580 - 640 nm), Red (Ex 625 nm: Em 660 - 720 nm), AFC (Ex 405 nm, Em 495 - 505 nm) |
| Detection Limit |
0.5 fmol/200 μl or 1 ppt of fluorescein in 96-well plate, 30 pg/well dsDNA with DNA Quantification Dye |
| Linear Dynamic Range |
6 logs, assay dependent |
| Calibration** |
Multipoint Calibration |
| Read Out |
Relative Fluorescence units, Direct Concentration |
| Discrete Sample Average |
Sample readings averaged over 0.5 seconds to improve accuracy |
| VISIBLE ABSORBANCE |
| Light Source |
LED |
| Detector |
Large-area photodiode |
| Spectral Range |
450 - 750 nm |
| Wavelengths for Installed Filters |
450, 560, 600, 750 nm, 2 slots for customization |
| Photometric Measuring Range |
0 - 5.0 OD |
| Linear Dynamic Range |
0 - 4.0 OD, assay dependent |
| OD Accuracy |
0.01 OD ± 3% at ≤ 2.5 OD |
| OD Precision |
0.01 OD ± 1% |
| Stray Light |
0.002% @ 560 nm in clear bottom, black wall plate |
| UV-VISIBLE ABSORBANCE |
| Light Source |
Xenon lamp |
| Detector |
Photodiode |
| Spectral Range |
200 - 1100 nm |
| Wavelengths for Installed Filters |
260, 280, 450, 560, 600, 750 nm (2 slots customizable) |
| Photometric Measuring Range |
0 - 4.0 OD |
| Linear Dynamic Range |
0 - 3.5 OD, assay dependent |
| OD Accuracy |
0.05 OD ± 3% at ≤ 2.5 OD |
| OD Precision |
0.002 ± 3% |
| Stray Light |
0.001% @ 560 nm in clear bottom, black wall plate |
*The GloMax®-Multi Microplate Multimode Reader uses 96-well plates only.
**Feature is not available on the GloMax®-Multi Microplate Multimode Reader.
***Data Output for the GloMax®-Multi Microplate Multimode Reader includes MAC compatible and .csv file format.
For research use only. Not for use in diagnostic procedures. GloMax is a trademark of Promega Corporation. All other trademarks are the sole property of their respective owners. For the most up-to-date specifications, visit www.promega.com.