Promega Corporation

Kazusa Collection

Explore 8,800+ expression-validated ORF clones

Kazusa DNA Research Institute (KDRI)

KDRI is an academic Japanese research institute dedicated to genome-scale studies and the function of individual genes. The Kazusa ORFeome Project was initiated to create a collection of human and mouse ORFs to enable the larger research community with a readily accessible, highly validated ORFeome. KDRI is an active member of the global ORFeome Collaboration, and are experts in HUGE (Human Unidentified Gene-Encoded) large proteins (>4kb). Promega and KDRI have established a partnership, allowing Promega customers access to Kazusa's coveted collection of experimentally validated clones.

Experimentally-Validated Collection

Kazusa understands the value of extensive validation, and provides this data to their customers for the HaloTag® ORF collection. Check out the representative data pieces below, which are available for nearly all HaloTag®-fusion ORFs before you order.

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Fluorescent Microscopy demonstrating in situ labeling and visualization of HaloTag® fusions with HaloTag® TMR Ligand.

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Expression Validation by SDS-PAGE analysis of expressed HaloTag®-fusions in HEK293 cell lysates

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Insert Size Confirmation through agarose gel electrophoresis

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Sequence Validation of both the 5' and 3' end sequences by single-pass sequencing

Overview of the Collection

Features HaloTag® Collection
PID beginning with FHC
Flexi® Collection
PID beginning with FXC
Size of Collection 8,872 human clones 6,396 human clones
Fusion Tag HaloTag® for protein purification, imaging and pull-downs Un-tagged
Validated Clones
Sequence Validated 100% clones
Insert Validated 99.8% clones 96% clones
Expression Validated 99.4% clones
Localization Validated 77.91% clones
Format DNA DNA
Typical Delivery 4-6 weeks 4-6 weeks

Gene Family Sets & Discounts Available

Allow us to quickly map your proteins of interest to the Kazusa collection to develop a custom set to meet your research needs. Send us an e-mail at proteomics@promega.com for pre-defined and custom gene family sets inquiries.

Gene Family Sets

  • Kinases
  • Tyrosine Kinases
  • MAP Kinases
  • G-Protein Coupled Receptors (G-PCRs)
  • Phosphatases
  • Transcription Factor-related Genes
  • Membrane Proteins
  • Protein Complexes
  • Cytokines & Chemokines
  • Ubiquitin Hydrolases (DUBS)
  • Ubiquitin Ligases (E1, E2, E3)
  • Tumor Suppressors
  • Oncogenes
  • Transporters and Pumps
  • Proteases

Research Areas

  • Epigenetics
  • Differentiation and Stem Cells
  • Cancer
  • Regulation
  • Signaling
  • Cell Cycle
  • Ion Channels
  • Apoptosis
  • DNA Repair
  • RNAi

Discounts are available for larger orders (excluded from additional promotions)

  • 10% discount by ordering 20-49 clones
  • 20% discount by ordering more than 50 clones

Inquire at proteomics@promega.com before placing an order.

Explore 8,800+ expression-validated ORF clones

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