MethylEdge® Bisulfite Conversion System
Rapid Bisulfite Conversion with Minimal DNA Fragmentation
- High-efficiency DNA conversion from as little as 100pg input DNA
- Rapid protocol saves time compared to other conversion systems
- Robust conversion of DNA with reduced DNA fragmentation
Catalog Number:
Size
Catalog Number: N1301
Bisulfite Conversion for DNA Methylation Workflows
The MethylEdge® Bisulfite Conversion System provides a rapid, efficient method to perform bisulfite conversion with minimal DNA fragmentation in less than two hours. The rapid protocol and complete conversion mean that you can produce completely converted DNA ready for your choice downstream assays with minimal preparation and hands-on time. Coupled with purification chemistries and flexible, robust amplification technologies for detection, the MethylEdge® Bisulfite Conversion System delivers high-quality, intact converted DNA for your experiments.
- High-efficiency DNA conversion
- Robust conversion of DNA with reduced DNA fragmentation
- Rapid Protocol
- Room-Temperature, Ready-to-Use Reagents
This system can be used for the bisulfite conversion and cleanup of DNA. Applications for converted DNA include bisulfite-specific PCR (BSP), bisulfite sequencing (bis-seq), bisulfite-specific high-resolution melt and COBRA analysis.
Rapid Conversion, Minimal Fragmentation
Realize the full potential of your samples with the most effective bisulfite conversion system available. The MethylEdge™ Bisulfite Conversion System offers a robust system for rapidly performing bisulfite conversion with minimal DNA fragmentation.
- High-efficiency DNA conversion from as little as 100pg input DNA
- Rapid Protocol saves time compared to other conversion systems
- Robust conversion of DNA with reduced DNA fragmentation
- Ready-to-use reagents stored at room temperature
Degree of DNA Fragmentation After Conversion
DNA Purification and Quantitation
Manual and High-Throughput Chemistries
ReliaPrep™ Genomic DNA Purification Systems offer comprehensive solutions for reliable purification of high-quality gDNA. Kits include manual systems for purification from FFPE samples, blood and tissue and high-throughput gDNA purification from large volume blood samples.
Hands-Free Automated Purification from 1–16 Samples
The Maxwell® RSC Instrument and DNA Purification Kits provide automated DNA extraction from many sample types, including FFPE tissues. The convenient, hands-free extraction method frees up time and provides consistent yields of highly pure gDNA.
Chemistry
The QuantiFluor™ dsDNA System offers sensitive detection of small amounts of double stranded DNA in solution upstream of bisulfite conversion.
Downstream Analysis
| End-Point PCR Systems | Dye-Based Real-Time Systems | Probe-Based Real-Time Systems |
|---|---|---|
|
GoTaq® Hot Start Master Mixes are premixed, ready-to-use solutions containing GoTaq® Hot Start Polymerase, magnesium, dNTPs and buffer. Reactions can be set up in less than a minute at room temperature; simply add your template, water and primers. |
GoTaq® qPCR Master Mix provides robust real-time PCR with earlier quantification cycle values and broad range detection for increased reliability, reproducibility and sensitivity. The system contains BRYT Green® dye, a novel fluorescent DNA-binding dye with minimal PCR inhibition for maximum efficiency, and greater fluorescence enhancement upon binding to double-stranded DNA than SYBR® Green I. |
The GoTaq® Probe qPCR Master Mix is optimized for quantitative PCR assays in the hydrolysis probe detection format. It is provided as a ready-to-use, stabilized 2X formulation that includes all components for qPCR (except template, primers and probe). |
Protocols
Complete Protocol
Protocolos Rápidos
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación |
|---|---|---|
Collection Tubes |
N124A | 1 × 50/pack |
ME Spin Columns |
N125A | 1 × 50/pack |
ME Elution Buffer |
N126A | 1 × 1ml |
ME Desulphonation Buffer |
N127A | 1 × 10ml |
ME Wash Buffer |
N128A | 1 × 6ml |
ME Binding Buffer |
N129A | 1 × 30ml |
ME Conversion Reagent |
N130A | 5 × 1.3ml |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
See Protocol for storage recommendations.
Use of Methylation-Specific PCR (MSP) is protected by U.S. Pat. Nos. 5,786,146, 6,017,704, 6,200,756 and 6,265,171. No license under these patents to use the MSP process is conveyed expressly or by implication to the purchaser by the purchase of this product.
Resources
No related resources available
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