Basics of Real-Time PCR Technologies (Three days)
This presentation of real-time polymerase chain reaction (PCR) theory and practice is ideal for nonscientists working in the field of Molecular Biology/Biotechnology or for scientists new to real-time PCR techniques who want to understand the fundamentals and gain laboratory experience.
Date: February 25-27, November 12-14, 2013
Additional dates can be arranged by contacting PETAL with a custom training request.
Daily schedule:
9:30 to 17:30 (21 hr)
Fees:
1400 € VAT excluded for researchers and technicians
1200 € VAT excluded for students
Who should attend:
This practical course is for scientists, teachers, laboratory technicians, researchers and others who would like to learn more about real-time PCR, analysis of results, optimizing reactions and troubleshooting problems.
Maximum number of attendees: 8
A certificate of completion is given to participants.
Course Content
This class is a three-day overview of real-time PCR technologies. Course topics include; brief overview of nucleic acid isolation, comparison between end-point and quantitative PCR (qPCR), review of existing qPCR techniques, setting up a SYBR® Green qPCR and multiplexing PCR.
| Lectures |
Laboratory |
| Nucleic acid isolation fundamentals |
Isolate and analyze nucleic acids for use in downstream reactions |
| Comparative review end-point PCR and real-time PCR techniques |
|
| Review of qPCR techniques |
|
| SYBR® Green PCR: Discuss primer design, optimization, data analysis and application to everyday experiments |
Use SYBR® dye to track amplification in real–time PCR |
| Multiplexing PCR: Learn how researchers amplify multiple gene products in a single reaction |
Fluorescent detection of multiple, amplified gene products |
Specific course objectives may vary depending on individual and group interests.
Please contact us if you have questions.
