Wheat Germ Extract

Wheat Germ Extract contains the cellular components necessary for protein synthesis (tRNA, ribosomes, initiation, elongation and termination factors). Wheat Germ Extract is prepared by grinding wheat germ in an extraction buffer followed by centrifugation to remove cell debris. The supernatant is subjected to chromatography that separates endogenous amino acids and plant pigments from the extract. The extract is also treated with micrococcal nuclease to destroy endogenous mRNA and thus reduce background translation to a minimum.

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Wheat Germ Extract contains the cellular components necessary for protein synthesis (tRNA, ribosomes, initiation, elongation and termination factors). Wheat Germ Extract is prepared by grinding wheat germ in an extraction buffer followed by centrifugation to remove cell debris. The supernatant is subjected to chromatography that separates endogenous amino acids and plant pigments from the extract. The extract is also treated with micrococcal nuclease to destroy endogenous mRNA and thus reduce background translation to a minimum.

Features - Benefits

• Optimized: Extract contains an energy-regenerating system (phosphocreatine/phosphocreatine kinase), spermidine (to stimulate the efficiency of chain elongation), magnesium acetate and potassium acetate.
• Flexible: Three Amino Acid Mixtures are provided, which enable different choices of radioisotopes.
• Robust: Potassium Acetate is provided to enhance translation for a wide range of mRNAs.
• Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/

Applications

• Expression of eukaryotic messages that do not express well in reticulocyte lysates.
• Expression of plant mRNAs.
• Expression of mammalian transcription factors.

For more information, see the Protein Expression chapter of the Protocols & Applications Guide.

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