Here we describe a two-part functional system for analyzing the
transcriptional and post-transcriptional regulation of genes. The
first part involves promoter-reporter constructs and protocols for measuring the
function of human promoter elements on a large
scale in living cells. The second part involves a similar concept using
reporter-UTR constructs to examine the functional effect of
UTR sequences on post-transcriptional regulation on a similar scale. Pairing the
two types of experiments for hundreds or
thousands of genes of interest will enable researchers to gain new insights into
the regulatory mechanisms behind gene expression
networks and pathways.
Cell Notes 21, 20–22.
Shelley Force Aldred and Nathan D. Trinklein