Detect Caspase-8 and -9 Activities Using the Homogeneous Caspase-Glo® Assays

We have developed sensitive and homogeneous luminescent methods for measuring caspase-8 and -9 activities, which represent a marked improvement over existing fluorescent-based methods. The assay sensitivity is sufficient to detect caspase-8 or -9 activity from the number of cells typically used in a 96-well format. The assays are also efficient means to screen for specific inhibitors of the caspases in biochemical assays. These robust and homogeneous assays are ideal tools for studying the role of the caspase cascade in apoptosis.

Cell Notes 8, 9–12.