Promega has developed a new, homogeneous reporter assay, the Dual-Glo® Luciferase Assay System, for monitoring both firefly and Renilla luciferases expressed in mammalian cells in 96- and 384-well plates. The new assay system integrates cell lysis and luminescence chemistry, and the luminescence kinetics have been extended over several hours. The homogeneous assay format allows dual-reporter assays to be more easily and rapidly performed by reducing sample processing requirements and eliminating the need for reagent injectors in luminometers.
Cell Notes 4, 14–15.
Erika Hawkins, Braeden Butler, Keith Wood, Michael O'Grady, Laurie Orr, and Michael Beck