Tfor Working with RNA and Troubleshooting Downstream Applications

High quality RNA is required for many molecular biology techniques. The isolation and use of purified RNA in the laboratory is complicated by the fact that, chemically and biologically, RNA is significantly more labile than DNA. The choice and optimization of RNA purification methods are important for successful isolation of quality RNA and consistent performance of downstream applications. In order to avoid damage or loss of RNA, caution should be exercised to avoid nuclease contamination of the RNA samples during preparation and handling.

Promega Notes 63, 17.