Pfu DNA Polymerase: A High Fidelity Enzyme for Nucleic Acid Amplification

Pfu DNA Polymerase exhibits the lowest error rate of any thermostable DNA polymerase studied. For routine PCR, where simple detection of an amplification product or estimation of the product's size is important, Taq DNA polymerase is the obvious enzyme to choose. However, when the amplified product is to be cloned, expressed or used in mutagenesis studies, Pfu DNA Polymerase is a much better enzyme of choice for PCR. Pfu DNA Polymerase is also used in blends with Taq DNA polymerase, or amino-terminally truncated versions of Taq DNA polymerase, to amplify longer stretches of DNA in PCR with greater accuracy than Taq DNA polymerase alone.

Promega Notes 68, 7.