Characterization of TnT® T7 Quick for PCR DNA

TnT® T7 Quick for PCR DNA is a rapid and convenient coupled transcription/translation system designed for expression of PCR-generated DNA templates. The system is quite robust and able to express a variety of proteins ranging in size from 10–150kDa. This article discusses the effects on expression of PCR product size and purity, AUG start codon context and the presence of a poly(A) tail.

Promega Notes 77, 19–22.