Promega Corporation

Protocols and Applications Guide

WHAT'S NEW

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Read the P&A Guide, watch video protocols and use the BioMath Calculators on your Android phone.

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Available for the iPhone and iPad. Try the new Restriction Enzyme Tool.

DOWNLOAD GUIDE

Download individual chapters using the links in each chapter or download the entire Guide using these links (14MB): Letter or A4.

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Each chapter includes:

  • Step-by-step protocols
  • Illustrations and example data
  • Extensive background information
  • Animated movies of key techniques and cellular events
  • Links to citations and other useful laboratory tools

GUIDE CONTENTS

  • PCR Amplification
    pcrapplications

    PCR Applications

    • Introductory animation about PCR
    • Optimization tricks
    • RT-PCR tips
    • Polymerase and transcriptase review
    • PCR & RT-PCR protocols
    • Troubleshooting
  • RNA Interference
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    RNA Interference

    • Learn how to design and optimize siRNA sequences
    • Use reporter genes for siRNA optimization
    • Investigate RNAi delivery strategies
    • View a Nature animation about the RNAi mechanism
  • Apoptosis
    apoptosis

    Apoptosis

    • Gather more data from a single sample with multiplexed assays
    • Use two or more methods to confirm apoptosis
    • Detect caspase activity with anti-PARP pAb
    • Learn about the molecular mechanisms of apoptosis
  • Cell Viability
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    Cell Viability

    • Compare assay methods based on your needs
    • Read about factors that effect viability assay outcome
    • Learn how to multiplex cell viability with cytotoxicity or reporter assays
    • Choose an assay with the Cell Viability Assistant
  • Protein Expression
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    Protein Expression

    • Optimize your protein yield
    • Learn about the gold standard: TNT® Systems
    • Use the product selector tool to choose your system
    • Explore eukaryotic, mammalian and bacterial systems
    • Read about non- radioactive labeling systems
  • Expression Analysis
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    Expression Analysis

    • Single gene or gene array systems
    • Tips for creating a ribonuclease-free environment
    • Recipes for common solutions
    • Total or polyA RNA isolation systems
  • Cell Signaling
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    Cell Signaling

    • Animations of MAPK and PI-3K pathways
    • High or low ATP concentration kinase asssays
    • Fluorescent, luminescent, radioactive and colorimetric kinase assays
    • Phosphorylation-specific antibodies for MAPK pathways
  • Bioluminescent Reporters
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    Bioluminescent Reporters

    • pGL4: The latest generation reporter vectors
    • Dual-luciferase system: for better control
    • Live-cell substrates
  • DNA Purification
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    DNA Purification

    • Tips to maximize yield and quality
    • Plasmid and genomic DNA purification
    • Transfection-quality miniprep DNA
    • High-, low- and medium-throughput
    • DNA from blood
    • Automated purification at your lab bench
  • Cell Imaging
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    Cell Imaging

    • HaloTag® Technology: One construct, multiple fluors
    • Live-cell imaging protocols
    • Rapid labeling and imaging
    • FACS®
  • Protein Purification and Analysis
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    Protein Purification and Analysis

    • Affinity tags for purification
    • Analyzing DNA:Protein interaction
    • HaloCHIP™ System
    • Pulldown assays for protein interactions
    • Recipes for commonly used solutions
  • Transfection
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    Transfection

    • Methods for achieving transfection
    • Optimizing transfection efficiency
    • Sample transfection protocol
    • Recipes for commonly used solutions
  • Cloning
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    Cloning

    • Learn about T-Vector cloning
    • Use Flexi® Vectors for easy sequence transfer from one vector to another
    • Get technical information on restriction enzymes
    • Find out what affects PCR cloning
    • Recipes for solutions used in cloning
  • DNA-Based Human Identification
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    DNA-Based Human Identification

    • Learn about how repetitive DNA is used for human identification applications such as forensic casework and relationship testing
    • Explore the steps required to generate a DNA profile
    • Gain information about automated key steps in the DNA-typing process
    • Discover links to Promega protocols, Profiles in DNA articles, validation guides and other resources
  • Buffers for Biochemical Reactions
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    Buffers for Biochemical Reactions

    • Explore how a buffer system works
    • Understand what makes a "good" buffer
    • Learn how to prepare buffers
    • Find recipes for common buffers
  • Epigenetics
    epigenetics

    Epigenetics

    • Explore epigenetic mechanisms of gene regulation
    • Discover how DNA methylation and histone modifications affect chromatin structure
    • Decipher the histone code
    • Learn about techniques to research epigenetics

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