Promega Corporation

Restriction Enzyme Capabilities at a Glance

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Restriction Enzyme Capabilities

A subset of Promega restriction enzymes offer capabilities that allow you to shorten your cloning workflow. These capabilities include rapid digestion of DNA in 15 minutes or less, ability to completely digest DNA directly in the GoTaq(R) Green Master Mix, and Blue/White Cloning Qualification which allows for rapid, reliable detection of transformants.

Restriction
Enzyme
Rapid Digest
Capable
GoTaq Buffer
Compatible
Blue/White
Cloning Qualified
AatII + +
Acc65I nt +
AccI + + +
AgeI + +
AluI + nt
ApaI nt + +
AvaI nt + +
BamHI + + +
BglII +
BstXI nt nt +
BstZI nt nt +
ClaI + + +
DdeI + nt
DpnI + nt
DraI nt +
Eco47III nt +
EcoICRI nt nt +
EcoRI + +
EcoRV + + +
HaeIII + nt
HincII nt + +
HindIII + + +
HpaI nt +
KpnI + + +
MluI nt + +
NcoI + + +
NdeI + +
NheI + +
NotI + nt +
NsiI nt + +
PstI + + +
PvuI +
PvuII + +
RsaI + nt
SacI nt nt +
SacII + +
SalI + + +
ScaI +
SfiI nt nt +
SgfI nt nt +
SmaI nt nt +
SpeI + nt +
SphI + + +
StuI nt +
XbaI + + +
XhoI + + +
XmaI + nt +

Rapid Digest Capable (+): Enzymes capable of digesting DNA in 15 minutes or less.

GoTaq® Buffer Compatible (+): Enzymes active and capable of digestion directly in 1X GoTaq® Green Master Mix and 1X PCR Master Mix.

Blue/White Cloning Qualified: Enzymes tested in specific subcloning reactions to assure the absence of interfering enzyme activities.

nt: Not Tested

–: Unsuccessful cutting or not qualified

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