GoTaq® MDx Hot Start Polymerase Product Information
Literature # 9PID600
GoTaq® MDx Hot Start Polymerase contains GoTaq® MDx Hot Start Polymerase, 5X Green GoTaq® Flexi Buffer, 5X Colorless GoTaq® Flexi Buffer and 25mM MgCl2. GoTaq® MDx Hot Start Polymerase contains the high-performance GoTaq® DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The polymerase activity is restored during the initial denaturation step when amplification reactions are heated at 94–95°C for two minutes. This allows hot-start PCR in which polymerase activity is inhibited at temperatures below 70°C, allowing convenient, room-temperature reaction setup. Hot-start PCR is advantageous for some amplification targets, because it may eliminate or minimize primer-dimer and secondary products. In some cases, hot-start PCR may improve yields. After activation, GoTaq® MDx Hot Start Polymerase exhibits 5′→3′ exonuclease activity.
The 5X Green GoTaq® Flexi Buffer contains two dyes (a blue dye and a yellow dye) that separate during electrophoresis to monitor migration progress. The colorless buffer is used when direct fluorescence or absorbance readings are required without prior purification of the amplified DNA from the PCR. The Flexi Buffers do not contain magnesium, allowing easy optimization in amplification reactions.