Caspase-Glo® 8 Assay Technical Bulletin

Literature # TB332

The Caspase-Glo® 8 Assay is a homogeneous luminescent assay that measures caspase-8 activity. The assay provides a luminogenic caspase-8 substrate in a buffer system optimized for caspase activity, luciferase activity and cell lysis. Addition of a single Caspase-Glo® 8 Reagent in an "add-mix-measure" format results in cell lysis, followed by caspase cleavage of the substrate and generation of a "glow-type" luminescent signal (produced by the luciferase reaction). The signal generated is proportional to the amount of caspase activity present.