Maxwell® 16 FFPE Tissue LEV DNA Purification Kit Technical Bulletin

Literature # TB382

The Maxwell® 16 FFPE Tissue LEV (Low-Elution Volume) DNA Purification Kit is used with the Maxwell® 16 Instrument (Cat.# AS2000) and LEV Hardware Kit (Cat.# AS1250) and is designed for optimal purification of DNA from one to ten sections (5µm) of formalin-fixed, paraffin-embedded (FFPE) tissue samples. To help minimize PCR inhibitor carryover, small amounts of DNA-binding paramagnetic particles are used, and as a result the DNA-binding capacity of the system is limited to a few hundred nanograms of pure DNA suitable for amplification applications including qPCR. However, tissue that has been stored in formalin for extended periods of time may be extensively cross-linked and/or too degraded for extraction of amplifiable DNA.