GoTaq® 2-Step RT-qPCR System Technical Manual

Literature # TM337

GoTaq® 2-Step RT-qPCR System is a reagent system for quantitative analysis of RNA using a two-step reverse transcription-quantitative PCR (RT-qPCR) protocol. The components and protocol allow cDNA synthesis using the GoScript™ Reverse Transcription System and quantification using the GoTaq® qPCR Master Mix. The GoTaq® 2-Step RT-qPCR System contains a new fluorescent DNA-binding dye that often exhibits greater fluorescence enhancement upon binding to double-stranded DNA (dsDNA) than SYBR® Green I. GoTaq® qPCR Master Mix can be used with any real-time instrument capable of detecting SYBR® Green I or FAM™ dye. GoTaq® qPCR Master Mix contains a low level of CXR reference dye. A separate tube of 100X CXR Reference Dye is included for use with instruments that require a higher level of reference dye than that in the GoTaq® qPCR Master Mix.