GoTaq® 1-Step RT-qPCR System Technical Manual

Literature # TM355

GoTaq® 1-Step RT-qPCR System is a reagent system for quantitative analysis of RNA using a one-step reverse transcription-quantitative PCR (RT-qPCR) protocol. The GoTaq® 1-Step RT-qPCR System combines the benefits of GoScript™ Reverse Transcriptase and GoTaq® qPCR Master Mix for efficient,sensitive and linear one-step RT-qPCR quantification over a wide range of RNA template inputs.

The GoTaq® 1-Step RT-qPCR System contains the proprietary fluorescent DNA-binding dye, BRYT Green® dye, that exhibits greater fluorescence enhancement upon binding to double-stranded DNA (dsDNA), than does SYBR® Green I.