Promega Corporation

Kinase-Glo® Luminescent Kinase Assay: Detect Virtually Any Kinase

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Abstract

The Kinase-Glo® Luminescent Kianse Assay is a homogeneous method for measuring kinase activity by quantifying a decrease in ATP levels following kinase activity. The assay can work with virtually any kinase and substrate combination, and it is performed in a single well of a standard multiwell plate by adding an equal volume of Kinase-Glo® Reagent and recording luminescence. The assay produces excellent  Z´-factor values, easily detects known kinase inhibitors, and provides accurate IC50 values.

Cell Notes 5, 5–8.

Richard Somberg, Beck Pferdehirt, and Kevin Kupcho

Promega Corporation
Publication Date: 2003

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