Promega Corporation

A Bioluminescent Assay for Calpain Activity

Share

  • Share
  • Print
  • Email
  • Download PDF

Abstract

We have developed an exceptionally sensitive assay for the proteases calpain I and II. The Calpain-Glo™ Protease Assay is a rapid, homogeneous assay with a luminescent signal that is proportional to the amount of calpain present in the sample. The Calpain-Glo™ Assay uses a recombinant thermostable luciferase, Ultra-Glo™ Luciferase, that dramatically improves sensitivity and detects calpain over a broad range of activity.

Promega Notes 91, 6–9.

Martha O’Brien1, Mike Scurria2, Kay Rashka1, Bill Daily2 and Terry Riss1

1Promega Corporation and 2Promega Biosciences, Inc.
Publication Date: 2005

Download Article (103 KB)

Prefer a different language?

Your country is set to Spain. Your language is set to español. Please select the language that will best suit your needs:

This is correct, continue to site »

I need additional help

It appears that you have Javascript disabled. Our website requires Javascript to function correctly. For the best browsing experience, please enable Javascript.