Assay Optimization and Profiling in uHTS Format Scientific Poster
Tracy Worzella1, Brad Larson1, Kevin Hsiao1 and Aoife Gallagher2
1Promega Corporation, 2800 Woods Hollow Road, Madison WI 53711, USA
2Deerac Fluidics, Unit 8 Enterprise Centre, Pearse Street, Dublin 2, Ireland
We demonstrate the use of Promega Kinase-Glo® Plus Luminescent Kinase Assay for miniaturized kinase assays using Deerac Fluidics Equator™ HTS liquid handling system. The Kinase-Glo® Plus Assay is a homogeneous method of measuring kinase activity by quantifying ATP in solution following a kinase reaction. The Equator™ HTS is a noncontact liquid dispenser capable of delivering volumes from 20μl down to 50nl.
Because assay development and screening are key uses for miniaturized assays, we show the Equator™ HTS can be used to optimize Kinase-Glo® reactions and screen for kinase activity. We use the Equator™ HTS to titrate kinases and substrates to determine optimal concentrations for subsequent screening activities in 1536-well format. A panel of kinases and compounds are counterscreened for kinase selectivity profiling. 24-point serial titration is performed to determine potency. Data show that miniaturization of the Kinase-Glo® Plus Assay does not compromise assay quality. The combination of Kinase-Glo® Plus and the Equator™ HTS provides an integrated solution for kinase drug discovery.
- Part# PS027
- Printed in USA.