Promega Corporation

Automation of Luminescence-Based CYP Inhibition Assays Scientific Poster

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Abstract

Brad Larson1, Peter Banks1, Jason Greene1, Sarah Shultz2, Jim Cali2, Mary Sobol2 and Simon Allard2
1BioTek Instruments, Inc., Winooski, VT
2Promega Corporation, Madison, WI

The ability to determine the effects that lead compounds have on cytochrome P450 enzymes is an important part of today's drug discovery routine. One desired component of this process is the ability to profile enzymes using the same basic procedure. The second is an easy, yet dependable way to dilute compounds that will create accurate titration curves. Here we demonstrate the automation of dose-response curves using BioTek's Precision™ XS automated pipetting system. IC50s of small molecule drugs were determined using recombinant CYP isoforms 3A4, 2C9 and 2D6 and luminogenic substrates specific to each from Promega's P450-Glo™ CYP450 Screening Systems. Compounds were profiled against all three isoforms on the same 384-well assay plate to demonstrate the ease of this combined procedure.

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  • Part# PS090
  • Printed in USA.

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