Sugino, T., Nozaki. K., Takagi, Y., Hattori, I., Hashimoto, N., Moriguchi, T., and Nishida, E.
Notes: The role of JNK, p38 and MAPK signaling pathways in delayed neuronal death after transient forebrain ischemia was examined using Western blotting and immunohistochemistry. Gerbil hippocampi were homogenized, subjected to SDS-PAGE, blotted to PVDF membranes and incubated with one of the following primary antibodies: Anti-ACTIVE® JNK pAb (1:2000 dilution), Anti-ACTIVE® p38 pAb (1:1000 dilution), and the Anti-ACTIVE® MAPK pAb (1:2000 dilution).The secondary antibody was the Donkey Anti-Rabbit IgG (H+L), AP, diluted 1:10,000 for JNK and p38 and 1:5000 for ERK. For immunohistochemistry, gerbils were perfused with 4% paraformaldehyde. Frozen 40µm sections were stained with either the Anti-ACTIVE® JNK pAb and the Anti-ACTIVE® p38 pAb. (2402)