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Clin. Can. Res. 7, 105-12. Nerve growth factor exerts differential effects on the growth of human pancreatic cancer cells. 2001

Zhu, Z.W., Friess, H., Wang, L., Bogardus, T., Korc, M., Kleeff, J., and Buchler M.W.

Notes: The levels of NGF in culture medium of numerous human pancreatic cancer cell lines was determined using Promega's NGF Emax® ImmunoAssay System. NGF-induced pancreatic cancer cell growth seemed to be dependent on the expression levels and the balance of the TrkA and p75 receptors. (2334)

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Neurosci. Lett. 280, 220-222.. Differential neurotrophin levels in cerebrospinal fluid and their changes during development in newborn rat. 2000

Xia, Y.X., Ikeda, T., Xia, X.Y., Ikenoue, T.

Notes: The levels of BDNF, NT-3 and NGF were examined in the cerebrospinal fluid of newborn rats (80 per sample) from postnatal day 1-21. NGF levels were consistently higher than the other neurotrophins ranging from 136pg/ml (day 21) to 607pg/ml (day 17) depending upon the day tested. NT-3 was the next most abundant ranging from approximately 40pg/ml (day 19) to 144pg/ml (day 7). BDNF was the least abundant ranging from 19.2pg/ml (day 5) to as high as 65pg/ml (day 13). NGF was determined with the NGF Emax® ImmunoAssay System (NGF ELISA), NT-3 levels were determined with the NT-3 Emax® ImmunoAssay System (NT-3 ELISA), and BDNF levels were determined with the BDNF Emax® ImmunoAssay System (BDNF ELISA). (0167)

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J. Neurosci. 20, 9174-9185. Neurotrophin-4/5 alters responses and blocks the effect of monocular deprivation in cat visual cortex during the critical period. 2000

Gillespie, D.C., Crair, M.C., and Stryker, M.P.

Notes: NT-4/5, NGF, and neurotrophin-3 (NT-3) was infused into cat primary visual cortex during a period of monocular deprivation. Cats were perfused transcardially with PBS, followed by 4% paraformaldehyde. The brain was embedded in gelatin-albumin in 50-80 µm sections. Sections were stained with Promega's Anti-Human NT-4/5, Anti-Human NT-3, or Anti-Human NGF Antibodies to determine the localization of these factors. (2369)

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Biotechnol. Appl. Biochem. 28, 215-218. An improved, inexpensive method for the large-scale purification of human nerve growth factor. 1998

Li, S., Li, F., Tan, W., Yang, N., Jin, H., Chen, H.

Notes: Human NGF was purified from term human placentas. The purified protein was recognized by the Anti-NGF mAb in a Western blot using the protocol in Neural Notes Vol.I Issue 1. (0798)

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Anti-NGF mAb

Eur. J. Immunol. 28, 3240-3251. Role of nerve growth factor in a mouse model of allergic airway inflammation and asthma. 1998

Braun, A., Appel, E., Baruch, R., Herz, U., Botchkarev, V., Paus, R., Brodie, C., Renz, H.

Notes: The NGF Emax® ImmunoAssay System was used to determine the NGF content of mouse lavage and serum as well as the conditioned media of mononuclear cells. Allergic conditions showed increases in NGF. (1400)

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