Optimizing RNA Expression Analysis from Start to Finish
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Presented by:
Dr. Doug Wieczorek. See speaker bio
Dr. Doug Wieczorek is a Senior Applications Scientist at Promega Corporation, Madison, WI. Dr. Wieczorek started his career at Promega in the Technical Services department before moving to the Scientific Applications group, which focuses on the development of new applications for existing products in response to customer requests. Prior to joining Promega, he received his Ph.D. in 2001 from the Interdisciplinary Graduate Program in Genetics at the University of Iowa where he studied viral and bacteriophage DNA packaging. He followed his thesis work with postdoctoral training in the Department of Microbiology at the University of Iowa as well as the Department of Genetics at the University of Wisconsin – Madison.
- Original Webinar Date: Tuesday, May 8, 2012 Tuesday, May 8, 2012
All Promega webinars are free, though we do ask you to register.
Optimizing RNA Expression Analysis from Start to Finish
ABSTRACT
RNA expression analysis is the foundation of many different research projects such as mechanistic studies of transcription, the identification cancer biomarkers and many more. As such, careful execution and characterization of each step in the RNA analysis workflow is required to produce high quality, reliable data you can trust. RNA expression analysis begins with the purification of high quality RNA, followed by total RNA quantitation and finally measurement of one or more specific target RNA transcripts, and numerous options are available at each of these steps. This webinar will review the purification, quantitation and target-specific RNA analysis methods available including advantages and disadvantages of each, as well as key considerations for successful completion of each step. Special emphasis will be given to total RNA quantitation, an often overlooked but critical step in RNA expression analysis.
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