MAO-Glo™ Assay Systems
Luminescent Monoamine Oxidase Assay
- Selectively determine MAO-A and MAO-B activity in cell-based or biochemical samples
- Luminescent assay is 100x more sensitive than fluorometric methods
- Simple "add and read" protocol ideal for high-throughput screening
Catalog Number:
Size
Catalog Number: V1401
Catalog Number: V1402
Selective and Sensitive Detection of Monoamine Oxidase Activity
The MAO-Glo™ Assay is performed by incubating the MAO enzyme source with a luminogenic MAO substrate. Upon reaction with MAO, the derivative is converted into luciferin, which in turn reacts with luciferase to produce light. The amount of light produced is directly proportional to the activity of MAO.
After the MAO reaction has been performed, the reconstituted Luciferin Detection Reagent is added. The reagent simultaneously stops the MAO reaction and initiates a stable glow-type luminescent signal with a half-life greater than 5 hours. This eliminates the need for strictly timed luminescent detection.
The MAO-Glo™ Assay includes a luminogenic MAO substrate, two MAO Reaction Buffers (one that can be used with either MAO A or MAO B enzyme and one that is designed specifically for MAO B), a lyophilized Luciferin Detection Reagent and the Luciferin Detection Buffer. The user supplies the sample material containing MAO. Protocols are configured for multiwell plate formats but easily can be adapted for single-tube applications.
MAO enzymes may be the key to treating various neurological conditions. Learn more in this blog: Monoamine Oxidase and Mental Health: From Psychedelics to Diet
Measure Monoamine Oxidase Inhibitor Activity with MAO-Glo
Screening for MAO Inhibitors and Substrates
See over 200 citations for the MAO-Glo™ Assay System.
Protocols
Complete Protocol
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación |
|---|---|---|
|
MAO Substrate |
V141A | 1 × 186μg |
|
MAO Reaction Buffer |
V142A | 1 × 10ml |
|
MAO B Reaction Buffer |
V143A | 1 × 10ml |
Reconstitution Buffer with Esterase |
V144A | 1 × 10ml |
|
Luciferin Detection Reagent |
V859A | 1 × 1 each |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación |
|---|---|---|
|
MAO Substrate |
V141B | 1 × 931μg |
|
MAO Reaction Buffer |
V142B | 1 × 50ml |
|
MAO B Reaction Buffer |
V143B | 1 × 50ml |
Reconstitution Buffer with Esterase |
V144B | 1 × 50ml |
|
Luciferin Detection Reagent |
V859B | 1 × 1 each |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
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