P450-Glo™ CYP3A4 Assay and Screening System
Luminescent Assays and Screening Systems for Measuring CYP3A4 Activity
- Screening systems include complete set of reagents
- Luminescent format eliminates need for time-consuming analyses
- Broad dynamic range and low background
Catalog Number:
Choose a luciferin substrate
Choose a product
Catalog Number: V9001
Catalog Number: V9920
Catalog Number: V9910
Catalog Number: V8911
Catalog Number: V8912
Catalog Number: V9002
The P450-Glo™ CYP3A4 Assays provide a homogeneous, luminescent method for measuring cytochrome P450 CYP3A4 activity. The assays are designed to measure the activities of P450s from recombinant and native sources and for testing the effects of analytes such as drugs and new chemical entities on P450 activities. These luminescent assays exhibit exquisite sensitivity, low background signals and broad dynamic range.
P450-Glo™ Assays employ luminogenic P450 substrates that are derivatives of beetle luciferin, a substrate for luciferase enzymes. The derivatives are not substrates for luciferase but are converted by P450s to luciferin, which in turn reacts with luciferase to produce light that is directly proportional to the activity of the P450.
The P450-Glo™ Assays generate a "glow-type" luminescent signal, produced using derivatized luciferins as P450 substrates and a recombinant stabilized luciferase (Ultra-Glo™ Luciferase) coupled with a proprietary buffer system. The half-life of the luminescent output is greater than two hours, eliminating the need for luminometers with injectors and allowing batch plate processing. The formulation also minimizes the incidence of false positives due to inhibition of luciferase by analytes when screening for cytochrome P450 inhibitors.
Dimethyl sulfoxide (DMSO), a common solvent used to solubilize chemical compounds, can significantly inhibit the activity of the 3A4 isoform of cytochrome P450, even at low concentrations (<0.1%).The P450-Glo™ CYP3A4 System (Luciferin-PPXE) DMSO-Tolerant Assay is specifically designed to tolerate DMSO in the 3A4 reaction. The assay exhibits little to no change in the signal-to-background ratio in the presence of 0.2% DMSO compared to a no-DMSO control.
Simple Protocol; Short Incubation Time
A variety of CYP3A4 systems to fit your research needs
P450-Glo™ CYP3A4 Assay and Screening System
P450-Glo™ CYP3A4 Assay and Screening System with Luciferin-IPA
P450-Glo™ CYP3A4 Assay and Screening System (Luciferin-PPXE) DMSO-Tolerant
Protocols
Complete Protocol
Protocolos Rápidos
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación |
|---|---|---|
Reconstitution Buffer with Esterase |
V144A | 1 × 10ml |
|
Luciferin-IPA |
V840A | 1 × 15μl |
|
Luciferin Detection Reagent |
V859A | 1 × 1 each |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
U.S. Pat. No. 8,288,559 and other patents pending.
Contenido
| Item | Part # | Presentación | Concentración |
|---|---|---|---|
Reconstitution Buffer with Esterase |
V144B | 1 × 50ml | |
|
Luciferin-Free Water |
V348A | 1 × 50ml | |
|
Potassium Phosphate Buffer, 1M |
V446A | 1 × 5ml | |
|
Control Membranes |
V476A | 1 × 100μl | 5mg/ml |
|
CYP3A4 (1pmol/μl) + Reductase + b5 |
V480B | 1 × 100μl | |
|
Luciferin-IPA |
V840B | 1 × 60μl | |
|
Luciferin Detection Reagent |
V859B | 1 × 1 each | |
|
Solution A—NADPH Regeneration System |
V952A | 1 × 2.75ml | |
|
Solution B—NADPH Regeneration System |
V953A | 1 × 600μl |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
U.S. Pat. No. 8,288,559 and other patents pending.
Contenido
| Item | Part # | Presentación | Concentración |
|---|---|---|---|
|
Luciferin-Free Water |
V348A | 1 × 50ml | |
|
2M Tris-HCl (pH 7.5), 20X |
V349A | 1 × 1ml | |
|
Potassium Phosphate Buffer, 1M |
V446A | 2 × 5ml | |
|
Control Membranes |
V476A | 1 × 100μl | 5mg/ml |
|
CYP3A4 (1pmol/μl) + Reductase + b5 |
V480A | 1 × 500μl | |
|
Luciferin Detection Reagent |
V859B | 1 × 1 each | |
|
Reconstitution Buffer |
V865B | 1 × 50ml | |
|
Solution A—NADPH Regeneration System |
V952A | 1 × 2.75ml | |
|
Solution B—NADPH Regeneration System |
V953A | 1 × 600μl | |
|
Luciferin-PPXE |
V997A | 2 × 15μl | 50mM |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
U.S. Pat. Nos. 7,692,022 and 8,106,052 and other patents pending.
U.S. Pat. No. 8,288,559 and other patents pending.
U.S. Pat. No. 8,592,172 and other patents pending.
U.S. Pat. No. 8,551,721 and other patents pending.
Contenido
| Item | Part # | Presentación | Concentración |
|---|---|---|---|
|
Luciferin Detection Reagent |
V859A | 1 × 1 each | |
|
Reconstitution Buffer |
V865A | 1 × 10ml | |
|
Luciferin-PPXE |
V997A | 1 × 15μl | 50mM |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
U.S. Pat. Nos. 7,692,022 and 8,106,052 and other patents pending.
U.S. Pat. No. 8,288,559 and other patents pending.
U.S. Pat. No. 8,592,172 and other patents pending.
U.S. Pat. No. 8,551,721 and other patents pending.
Contenido
| Item | Part # | Presentación | Concentración |
|---|---|---|---|
|
Luciferin Detection Reagent |
V859B | 1 × 1 each | |
|
Reconstitution Buffer |
V865B | 1 × 50ml | |
|
Luciferin-PPXE |
V997A | 2 × 15μl | 50mM |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
U.S. Pat. Nos. 7,692,022 and 8,106,052 and other patents pending.
U.S. Pat. No. 8,288,559 and other patents pending.
U.S. Pat. No. 8,592,172 and other patents pending.
U.S. Pat. No. 8,551,721 and other patents pending.
Contenido
| Item | Part # | Presentación |
|---|---|---|
Reconstitution Buffer with Esterase |
V144B | 1 × 50ml |
|
Luciferin-IPA |
V840B | 1 × 60μl |
|
Luciferin Detection Reagent |
V859B | 1 × 1 each |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
U.S. Pat. No. 8,288,559 and other patents pending.
Resources
Artículos
Otros
- Assays for Biochemical Targets
- Application Note: Measuring P450-Glo® Assays on the GloMax® Discover System
- Application Note: P450‐Glo™ CYP3A4 Assay on 3D microtissues
- Blog Article: Cytochrome P450 Inhibition: Old Drug, New Tricks
- Blog Article: Screening for Drug-Drug Interactions with PXR and CYP450 3A4 Activation
Related Products
Productos Similares
P450-Glo™ CYP1B1 Assay System
Mida la actividad del CYP1B1 con este ensayo luminiscente.
V8761, V8762
P450-Glo™ CYP2C8 Assay System
Mida la actividad del CYP2C8 con este ensayo luminiscente.
V8781, V8782
P450-Glo™ CYP1A1 Assay System
Mida la actividad del CYP1A1 con este ensayo luminiscente.
V8751, V8752
P450-Glo™ CYP1A2 Assay and Screening Systems
Mida la actividad del CYP1A2 con este ensayo luminiscente, o elija el sistema de cribado del CYP1A2 para obtener un set completo de reactivos.
V8771, V8772, V8421, V8422, V9770
P450-Glo™ CYP2C19 Assay and Screening Systems
Mida la actividad de CYP2C19 con este ensayo luminiscente, o elija el sistema de cribado para obtener un set completo de reactivos.
V8881, V8882, V9880
P450-Glo™ CYP2C9 Assay and Screening Systems
Mida la actividad de CYP2C9 con este ensayo de luminiscencia, o elija el sistema de cribado para obtener un set completo de reactivos.
V8791, V8792, V9790
P450-Glo™ CYP2D6 Assay and Screening Systems
Mida la actividad del CYP2D6 con este ensayo luminiscente, o elija el sistema de cribado para un set completo de reactivos.
V8891, V8892, V9890
Usado con frecuencia junto con
Apo-ONE® Homogeneous Caspase-3/7 Assay
Ensayo fluorescente sensible que permite medir la actividad de las caspasa-3 y -7 activas.
G7792, G7790, G7791
Caspase-Glo® 3/7 Assay System
Ensayo luminiscente fácil de usar que permite detectar la actividad de la caspasa-3/7.
G8090, G8091, G8093, G8092, MG1010
