CDK7/CyclinH1/MNAT1 Kinase Enzyme System

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The CDK7/CyclinH1/MNAT1 Kinase Enzyme System contains:

• CDK7/CyclinH1/MNAT1 Kinase, 10μg (Human, full-length recombinant). MW: ~40kDa CDK7, ~39kDa CyclinH1, ~37kDa MNAT1
• Native Swine Myelin Basic Protein (MBP) Substrate, (1mg/ml)
• 5X Reaction Buffer, 0.1M DTT

Recombinant full-length human CDK7, Cyclin H1 and MNATI were co-expressed by baculovirus in Sf9 insect cells using N-terminal His tags. CDK7 gene is a member of the cyclin-dependent protein kinase family that is important regulators of cell cycle progression. CDK7 forms a trimeric complex with cyclin H and MAT1, which functions as a CDK-activating kinase (CAK). CDK7 is an essential component of the transcription factor TFIIH that is involved in transcription initiation and DNA repair. CDK7 is thought to serve as a direct link between the regulation of transcription and the cell cycle.

Application Note

CDK7 NCBI Database Entry
CyclinH1 NCBI Database Entry
MNAT1 NCBI Database Entry

The CDK7/CyclinH1/MNAT1 Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo™ Kinase Assay

The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.