DGKB Kinase Enzyme System

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The DGKB Kinase Enzyme System contains:

• DGKB Kinase, 10μg (Human, recombinant, full-length); MW: ~118kDa
• Dilauroyl-sn-glycerol Substrate, 10mM 
• 5X Reaction Buffer; DTT, 0.1M; Lipid Dilution Buffer II; CaCl₂ Solution, 1M

Recombinant full-length human DGKB was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Diacylglycerol kinase beta (DAG kinase beta) is a member of class I diacylglycerol kinase family and is regulated by Ca²⁺. Via converting diacylglycerol into phosphatidic acid, diacylglycerol kinases decrease the diacylglycerol levels to regulate the activity of PKC, Rho and Ras families. DGK beta is localized in brain and is involved in emotion and long-term memory linked to cognitive function.

Application Note 

NCBI Database Entry

The DGKB Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo™ Kinase Assay

The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.