ADP-Glo™ Kinase Assay with Lipid Kinase Substrates
Substrate and Assay Reagents for Detecting Class I PI3K Activity
- Use in screening assays with purified PI3K Class I lipid kinases
- Simple, scalable "add-and-read" assay format
Catalog Number:
Choose a substrate
Size
Catalog Number: V1781
Catalog Number: V1782
Catalog Number: V1791
Catalog Number: V1792
Ready-to-Use Lipid Kinase Substrates and Detection Reagents
The ADP-Glo™ Kinase Assay with PI:3PS (Cat.# V1781, V1782) contains PI:3PS Lipid Kinase Substrate (0.25mg) and the ADP-Glo™ Kinase Assay.
The ADP-Glo™ Kinase Assay with PIP2:3PS (Cat.# V1791, V1792) contains PIP2:3PS Substrate (0.25mg) and the ADP-Glo™ Kinase Assay.
These products are designed for use in screening assays with purified PI3K Class I lipid kinases. The lipid substrates are supplied as frozen small unilamellar vesicles containing a mixture of phosphatidylinositol (PI) or phosphoinositol-4,5-bisphosphate (PIP2) at a 1:3 ratio with phosphatidylserine (PS) as carrier lipid. The PIP2:3PS substrate is optimized for use with class I PI3Ks. The PI:3PS substrate provides a universal PI lipid kinase substrate.
The ADP-Glo™ Kinase Assay with PI:3PS and the ADP-Glo™ Kinase Assay with PIP2:3PS are components of the PI3K-Glo™ Class I Profiling Kit (Cat.# V1690).
Lipid Kinase Assay Principle
The lipid kinase reaction is performed by incubating lipid substrate (PI:3PS or PIP2:3PS) with a recombinant enzyme and ATP, and kinase activity is measured using the ADP-Glo™ Kinase Assay. After the kinase reaction, an ATP-depletion reagent is added to terminate the lipid kinase reaction and deplete any remaining ATP, leaving only ADP. Next, a detection reagent is added to simultaneously convert ADP to ATP, which is converted to a luminescent signal using a coupled luciferase/luciferin reaction.
Protocols
Complete Protocol
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
| PI:3PS Substrate, 1mg/ml PI | V171A | 1 × 0.5ml | View Product |
| Lipid Dilution Buffer, 10X | V180A | 1 × 1ml | |
|
MgCl2, 1M |
V330A | 1 × 1ml | |
|
ADP-Glo™ Reagent |
V912A | 1 × 5ml | |
|
Kinase Detection Buffer |
V913A | 1 × 10ml | |
|
Kinase Detection Substrate |
V914A | 1 × 1 each | |
|
Ultra Pure ATP, 10mM |
V915A | 1 × 500μl | |
|
ADP, 10mM |
V916A | 1 × 500μl |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
See Technical Manual for detailed storage recommendations.U.S. Pat. No. 7,700,310 and other patents and patents pending.
U.S. Pat. No. 8,183,007 and other patents and patents pending.
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
|
ADP-Glo™ Reagent |
V912B | 1 × 50ml | |
|
Kinase Detection Buffer |
V913B | 1 × 100ml | |
|
Kinase Detection Substrate |
V914B | 1 × 1 each | |
|
Ultra Pure ATP, 10mM |
V915B | 1 × 5ml | |
|
ADP, 10mM |
V916B | 1 × 5ml | |
| PI:3PS Substrate, 1mg/ml PI | V171A | 10 × 0.5ml | View Product |
| Lipid Dilution Buffer, 10X | V180A | 5 × 1ml | |
|
MgCl2, 1M |
V330A | 1 × 1ml |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
See Technical Manual for detailed storage recommendations.U.S. Pat. No. 7,700,310 and other patents and patents pending.
U.S. Pat. No. 8,183,007 and other patents and patents pending.
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
| PIP2:3PS Substrate, 1mg/ml PIP2 | V170A | 1 × 0.25ml | View Product |
| Lipid Dilution Buffer, 10X | V180A | 1 × 1ml | |
|
MgCl2, 1M |
V330A | 1 × 1ml | |
|
ADP-Glo™ Reagent |
V912A | 1 × 5ml | |
|
Kinase Detection Buffer |
V913A | 1 × 10ml | |
|
Kinase Detection Substrate |
V914A | 1 × 1 each | |
|
Ultra Pure ATP, 10mM |
V915A | 1 × 500μl | |
|
ADP, 10mM |
V916A | 1 × 500μl |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
See Technical Manual for detailed storage recommendations.U.S. Pat. No. 7,700,310 and other patents and patents pending.
U.S. Pat. No. 8,183,007 and other patents and patents pending.
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
|
ADP-Glo™ Reagent |
V912B | 1 × 50ml | |
|
Kinase Detection Buffer |
V913B | 1 × 100ml | |
|
Kinase Detection Substrate |
V914B | 1 × 1 each | |
|
Ultra Pure ATP, 10mM |
V915B | 1 × 5ml | |
|
ADP, 10mM |
V916B | 1 × 5ml | |
| PIP2:3PS Substrate, 1mg/ml PIP2 | V170A | 10 × 0.25ml | View Product |
| Lipid Dilution Buffer, 10X | V180A | 5 × 1ml | |
|
MgCl2, 1M |
V330A | 1 × 1ml |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
See Technical Manual for detailed storage recommendations.U.S. Pat. No. 7,700,310 and other patents and patents pending.
U.S. Pat. No. 8,183,007 and other patents and patents pending.
Resources
No related resources available
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