NUAK1 Kinase Enzyme System

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The NUAK1 Kinase Enzyme System contains:

• NUAK1 Kinase, 10μg (Human, recombinant; full-length); MW: ~122kDa
• CHKtide Substrate (1mg/ml; KKKVSRSGLYRSPSMPENLNRPR); derived from human CDC25C protein isoform A (amino acids 205–225)
• 5X Reaction Buffer; DTT, 0.1M

NUAK1 Kinase Enzyme System, (recombinant full-length human; ARK5) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. NUAK1 or SNF1/AMP kinase-related kinase (SNARK) is a member of the NUAK family of SNF1-like kinase 1 that is also known as AMPK-related protein kinase 5 (ARK5). ARK5 is a tumor cell survival factor that is activated by AKT and acts as an ATM kinase under conditions of nutrient starvation.

Application Note 

NCBI Database Entry 

The NUAK1 Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo™ Kinase Assay

The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.