PLK2 Kinase Enzyme System

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The PLK2 Kinase Enzyme System contains:

• PLK2 Kinase, 10μg (Human, full-length recombinant). MW: ~106kDa
• Native Bovine Casein, dephosphorylated (1mg/ml)
• 5X Reaction Buffer, 0.1M DTT

Full-length recombinant human PLK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PLK2 or Polo-like kinase 2 is a serum-inducible kinase that is a member of the Polo family of serine/threonine protein kinases, which play an essential role in normal cell division. PLK2 phosphorylates the P4.1-associated protein (CPAP), a human homologue of SAS-4, in procentriole formation during the centrosome cycle.

Application Note

NCBI Database Entry

The PLK2 Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo™ Kinase Assay

The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.