RSK4 Kinase Enzyme System

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The RSK4 Kinase Enzyme System contains:

• RSK4 Kinase, 10μg (Human, recombinant, full-length); MW: ~112kDa
• RSK Substrate (1mg/ml; KRRRLSSLRA); derived from human 40S ribosomal protein S6 (amino acids 230–239)
• 5X Reaction Buffer; 0.1M DTT

Recombinant full-length human RSK4 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. RSK4 is a member of the 90kDa ribosomal S6 kinase family which are important components of growth factor mediated stimulation of cellular proliferation, survival and differentiation. RSK4 is activated via coordinated phosphorylation by ERK and 3-phosphoinositide-dependent protein kinase-1 (PDK1). RSK4 is often associated with contiguous gene syndromes consisting of X-linked deafness type 3 (DFN3), mental retardation (MRX) and choroideremia (CHM). RSK4 is most abundantly expressed in the brain and kidney.  

Application Note 

NCBI Database Entry

The RSK4 Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo™ Kinase Assay

The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.