Intracellular TE Nano-Glo® Substrate/Inhibitor
For use with NanoBRET® Target Engagement Intracellular Assays
- Supplies the NanoBRET® Nano-Glo® Substrate and Extracellular NanoLuc® Inhibitor
- Optimal for experiments ≤2 hours
- Ensures measurements originate from inside intact cells
Catalog Number:
Size
Catalog Number: N2162
Catalog Number: N2160
Catalog Number: N2161
The Intracellular TE Nano-Glo® Substrate/Inhibitor is used for Intracellular NanoBRET® Target Engagement (TE) assays. The Intracellular TE Nano-Glo® Substrate/Inhibitor contains two components: the NanoBRET® Nano-Glo® substrate and the Extracellular NanoLuc® Inhibitor. The NanoBRET® Nano-Glo® Substrate is the substrate used by NanoLuc® Luciferase to generate the donor signal in several NanoBRET® Assays. The Extracellular NanoLuc® Inhibitor is used to suppress any NanoLuc® signal outside cells. The inhibitor thereby ensures that the NanoBRET® measurements originate from inside intact cells.
The Intracellular TE Nano-Glo® Substrate/Inhibitor is available separately to supplement the following systems:
- NanoBRET® Target Engagement HDAC Assays
- NanoBRET® Target Engagement BET BRD Assays
- NanoBRET® Target Engagement Kinase Assays
In addition, the Intracellular TE Nano-Glo® Substrate/Inhibitor is used with Intracellular NanoBRET® Target Engagement Tracers that are made using NanoBRET® Dyes (which can be obtained from our Custom Assay Services). For additional information, please enquire.
Measure Kinase-Compound Affinity in Live Cells
Learn More about NanoBRET® Kinase Target EngagementSpecifications
Catalog Number:
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
NanoBRET® Nano-Glo® Substrate |
N157A | 1 × 50μl | View Product |
Extracellular NanoLuc® Inhibitor |
N235A | 1 × 17μl | View Product |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
NanoBRET® TE LAR Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than genetically encoded autofluorescent protein, researcher must:
(i) use NanoBRET®-branded energy acceptors (e.g., NanoBRET® tracers, NanoBRET® dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
NanoBRET® Nano-Glo® Substrate |
N157C | 1 × 330μl | View Product |
Extracellular NanoLuc® Inhibitor |
N235B | 1 × 110μl | View Product |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
NanoBRET® TE LAR Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than genetically encoded autofluorescent protein, researcher must:
(i) use NanoBRET®-branded energy acceptors (e.g., NanoBRET® tracers, NanoBRET® dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
NanoBRET® Nano-Glo® Substrate |
N157D | 1 × 3.3ml | View Product |
Extracellular NanoLuc® Inhibitor |
N235C | 1 × 1.1ml | View Product |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
NanoBRET® TE LAR Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than genetically encoded autofluorescent protein, researcher must:
(i) use NanoBRET®-branded energy acceptors (e.g., NanoBRET® tracers, NanoBRET® dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
Resources
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