pGEM®-3Zf(+/–) Vectors
Standard Cloning Vectors That Can Produce ssDNA and In vitro Transcribe Sense and Antisense RNA
- Blue/white screening for easy identification of recombinant clones
- In vitro transcription using the SP6 and T7 RNA polymerase promoters flanking the multiple cloning region
- The difference between + and – vectors is orientation of the f1 origin
Catalog Number:
Choose a vector
Size
Catalog Number: P2271
Catalog Number: P2261
The pGEM®-3Zf(+) and pGEM®-3Zf(–) Vectors contain T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region contains unique restriction sites for EcoRI, SacI, KpnI, AvaI, SmaI, BamHI, XbaI, SalI, AccI, HincII, PstI, SphI and HindIII. The pGEM®-3Zf(+) and -3Zf(–) Vectors can be used as standard cloning vectors and as templates for in vitro transcription.
Reference
- Yanisch-Perron, C. et al. (1985) Gene 33, 103–19.
pGEM®-3Zf(+) Vector GenBank® Accession Number X65306 and vector sequence text file.
pGEM®-3Zf(–) Vector GenBank® Accession Number X65307 and vector sequence text file.
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación |
|---|---|---|
|
pGEM®-3Zf(+) Vector |
P227A | 1 × 20μg |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
See Protocol for storage recommendations.
Contenido
| Item | Part # | Presentación |
|---|---|---|
|
pGEM®-3Zf(–) Vector |
P226A | 1 × 20μg |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
See Protocol for storage recommendations.
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