BamHI
- Capable of digesting DNA in 15 minutes or less
- Active and capable of digestion directly in GoTaq® Green Master Mix
- Blue/white cloning-qualified, providing a higher level of quality control for enzymes used in cloning applications
Catalog Number:
Choose a concentration
Size
Catalog Number: R6021
Cut Site:
G▼GATC C
C CTAG▲G
Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/
Usage Notes:
- Star activity may be observed with glycerol concentrations >12%, enzyme:DNA ratio >25u/μg, low salt conditions or in the presence of Mn2+.
- Ends generated with BamHI can be directly ligated to ends generated with BglII, BclI and XhoII. These restriction sites are not regenerated in the ligation product.
Incubation Conditions: Buffer E. 37°C.
Source: Bacillus amyloliquefaciens H.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 5mM MgCl2, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
Percent Activity in 4-CORE® Buffer:
| A | B | C | D | E | MULTI-CORE™ |
|---|---|---|---|---|---|
| 75–100%* | 75–100% | 75–100% | 50–75% | 100% | 75–100% |
*Buffer A is not recommended due to potential star activity.
Frequency of Cutting:
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
|---|---|---|---|---|---|
| 5 | 3 | 0 | 1 | 1 | 1 |
Protocols
Complete Protocol
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación | Concentración | Available Separately |
|---|---|---|---|---|
|
Buffer E 10X Buffer |
R005A | 1 × 1ml | ||
|
Bovine Serum Albumin, Acetylated |
R396D | 1 × 150μl | 10mg/ml | View Product |
|
BamHI |
R602A | 1 × 2,500u | ||
|
MULTI-CORE™ 10X Buffer |
R999A | 1 × 0.25ml | View Product |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Resources
Related Products
Productos Similares
Usado con frecuencia junto con
Blue/Orange Loading Dye, 6X
Colorante para cargar muestras de ADN en pocillos de geles de electroforesis y seguir la migración durante esta.
G1881
LigaFast™ Rapid DNA Ligation System
Efficiently ligates sticky-ended DNA inserts into plasmid vectors in just 5 minutes at room temperature (blunt-ended inserts in as little as 15 minutes).
M8221, M8225
