BglI

Catalog Number:

Choose a concentration

10u/µl

Size

1,000u

Catalog Number: R6071

Cut Site:

GCCN NNN^▼NGGC
CGGN_▲NNN NCCG

Notes:

BglI recognizes the defined internal six bases of the SfiI recognition site and also cuts at the same position.
For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.

Incubation Conditions: Buffer D. 37°C.

Source: Bacillus globigii.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

A	B	C	D	MULTI-CORE™
10–25%	25–50%	75–100%	100%	100%

Frequency of Cutting:

λ	Ad-2	ΦX174	pUC18	M13mp18	pBR322
29	20	0	2	1	3

Protocols

Catalog Number:

Item	Part #	Presentación	Concentración	Available Separately
Buffer D 10X Buffer	R004A	1 × 1ml
Bovine Serum Albumin, Acetylated	R396D	1 × 150μl	10mg/ml	View Product
BglI	R607A	1 × 1,000u
MULTI-CORE™ 10X Buffer	R999A	1 × 0.25ml		View Product