SacI

Cut Site:

G  AGCT^▼C
C_▲TCGA  G

Note: SacI recognizes the same DNA sequence but cleaves at a different nucleotide than its neoschizomer, EcoICRI.

Incubation Conditions: Buffer J. 37°C.

Source: Streptomyces achromogenes.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

Frequency of Cutting: