TaqI

Cut Site:

T^▼CG  A
A  GC_▲T

Note: Sensitive to overlapping dam methylation.

Incubation Conditions: Buffer E. 65°C. (TaqI exhibits <10% activity at 37°C.)

Source: Thermus aquaticus YTI.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

Frequency of Cutting: